A de novo philadelphia chromosome-positive acute mixed-lineage leukemia with both major and minor BCR/ABL mRNA transcripts

Am J Hematol. 2000 Sep;65(1):72-4. doi: 10.1002/1096-8652(200009)65:1<72::aid-ajh13>3.0.co;2-r.

Abstract

A patient with a Philadelphia chromosome (Ph)-positive acute mixed-lineage leukemia (AMLL) expressing both major and minor BCR/ABL mRNA transcripts is described. Phenotypic analysis of the leukemic blasts revealed positivity for both myeloid and B-cell lineages. Southern blot analysis showed a rearrangement of the immunoglobulin heavy chain (IgH) gene. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis showed the expression of both major and minor BCR/ABL mRNA transcripts. To our knowledge, this is the first report of AMLL expressing both major and minor BCR/ABL mRNA transcripts and rearrangement of the IgH gene.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Antineoplastic Combined Chemotherapy Protocols / administration & dosage
  • Blotting, Southern
  • Bone Marrow Cells / pathology
  • Burkitt Lymphoma / drug therapy
  • Burkitt Lymphoma / genetics*
  • Fusion Proteins, bcr-abl / genetics*
  • Gene Rearrangement
  • Histocytochemistry
  • Humans
  • Immunoglobulin Heavy Chains / genetics
  • Leukemia, Myeloid, Acute / drug therapy
  • Leukemia, Myeloid, Acute / genetics*
  • Male
  • Peroxidase / analysis
  • Philadelphia Chromosome*
  • RNA, Messenger / analysis*
  • Remission Induction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Translocation, Genetic

Substances

  • Immunoglobulin Heavy Chains
  • RNA, Messenger
  • Peroxidase
  • Fusion Proteins, bcr-abl