Abstract
A retroviral vector containing wild-type p53 tumor suppressor gene (wt-p53) under the control of viral LTR sequences was constructed and transfected into packaging cell line GP+envAm12. Virus producing single cell clone GP+envAm12/ p53clC8 (8 x 10(5) cfu/ml, determined on NIH 3T3 cells) was isolated and used to transfer wt-p53 gene into human glioma cell lines in vitro. Decreased viability in p53-infected cells as compared to uninfected or empty virus infected cells was observed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells / virology
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Animals
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Antineoplastic Combined Chemotherapy Protocols / pharmacology
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Cell Survival / genetics
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Cisplatin / administration & dosage
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Doxorubicin / administration & dosage
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Etoposide / administration & dosage
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Genes, p53 / genetics*
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Genetic Therapy
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Genetic Vectors / genetics
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Genetic Vectors / pharmacology*
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Glioma / drug therapy
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Glioma / genetics
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Glioma / therapy*
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Humans
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Mice
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Retroviridae / genetics*
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Transfection / methods*
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Tumor Suppressor Protein p53 / biosynthesis
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Tumor Suppressor Protein p53 / genetics
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Tumor Suppressor Protein p53 / physiology
Substances
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Tumor Suppressor Protein p53
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Etoposide
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Doxorubicin
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Cisplatin