Abstract
The fibroblast growth factor receptor (FGFR)2 gene has been shown to be amplified in 5-10% of breast cancer patients. A breast cancer cell line developed in our laboratory, SUM-52PE, was shown to have a 12-fold amplification of the FGFR2 gene, and FGFR2 message was found to be overexpressed 40-fold in SUM-52PE cells as compared with normal human mammary epithelial (HME) cells. Both human breast cancer (HBC) cell lines and HME cells expressed two FGFR2 isoforms, whereas SUM-52PE cells overexpressed those two isoforms, as well as several unique FGFR2 polypeptides. SUM-52PE cells expressed exclusively FGFR2-IIIb isoforms, which are high-affinity receptors for fibroblast growth factor (FGF)-1 and FGF-7. Differences were identified in the expression of the extracellular Ig-like domains, acid box and carboxyl termini, and several variants not previously reported were isolated from these cells.
MeSH terms
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Alternative Splicing
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Breast Neoplasms / genetics
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Breast Neoplasms / metabolism
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Breast Neoplasms / pathology*
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Cell Line
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Cloning, Molecular
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Exons / genetics
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Female
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Fibroblast Growth Factor 1
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Fibroblast Growth Factor 2 / metabolism
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Fibroblast Growth Factor 7
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Fibroblast Growth Factors / metabolism
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Gene Amplification
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Gene Expression Regulation, Neoplastic*
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Humans
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Neoplasm Proteins / biosynthesis*
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Neoplasm Proteins / genetics
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Protein Isoforms / biosynthesis*
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Protein Isoforms / genetics
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA, Neoplasm / genetics
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RNA, Neoplasm / metabolism
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Receptor Protein-Tyrosine Kinases / biosynthesis*
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Receptor Protein-Tyrosine Kinases / genetics
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Receptor, Fibroblast Growth Factor, Type 2
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Receptors, Fibroblast Growth Factor / biosynthesis*
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Receptors, Fibroblast Growth Factor / genetics
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Recombinant Fusion Proteins / biosynthesis
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Reverse Transcriptase Polymerase Chain Reaction
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Substrate Specificity
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Transfection
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Tumor Cells, Cultured / metabolism*
Substances
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FGF7 protein, human
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Neoplasm Proteins
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Protein Isoforms
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RNA, Messenger
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RNA, Neoplasm
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Receptors, Fibroblast Growth Factor
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Recombinant Fusion Proteins
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Fibroblast Growth Factor 2
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Fibroblast Growth Factor 1
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Fibroblast Growth Factor 7
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Fibroblast Growth Factors
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FGFR2 protein, human
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Receptor Protein-Tyrosine Kinases
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Receptor, Fibroblast Growth Factor, Type 2