The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G(2) phase

M Izumi; K Yanagi; T Mizuno; M Yokoi; Y Kawasaki; K Y Moon; J Hurwitz; F Yatagai; F Hanaoka

doi:10.1093/nar/28.23.4769

The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G(2) phase

Nucleic Acids Res. 2000 Dec 1;28(23):4769-77. doi: 10.1093/nar/28.23.4769.

Authors

M Izumi¹, K Yanagi, T Mizuno, M Yokoi, Y Kawasaki, K Y Moon, J Hurwitz, F Yatagai, F Hanaoka

Affiliation

¹ Division of Radioisotope Technology, Cellular Physiology Laboratory, RIKEN (The Institute of Physical and Chemical Research), Wako, Saitama 351-0198, Japan.

Abstract

Mcm10 (Dna43), first identified in Saccharomyces cerevisiae, is an essential protein which functions in the initiation of DNA synthesis. Mcm10 is a nuclear protein that is localized to replication origins and mediates the interaction of the Mcm2-7 complex with replication origins. We identified and cloned a human cDNA whose product was structurally homologous to the yeast Mcm10 protein. Human Mcm10 (HsMcm10) is a 98-kDa protein of 874 amino acids which shows 23 and 21% overall similarity to Schizosaccharomyces pombe Cdc23 and S. cerevisiae Mcm10, respectively. The messenger RNA level of HsMcm10 increased at the G(1)/S-boundary when quiescent human NB1-RGB cells were induced to proliferate as is the case of many replication factors. HsMcm10 associated with nuclease-resistant nuclear structures throughout S phase and dissociated from it in G(2) phase. HsMcm10 associated with human Orc2 protein when overexpressed in COS-1 cells. HsMcm10 also interacted with Orc2, Mcm2 and Mcm6 proteins in the yeast two-hybrid system. These results suggest that HsMcm10 may function in DNA replication through the interaction with Orc and Mcm2-7 complexes.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
COS Cells
Cell Cycle / genetics
Cell Cycle Proteins / genetics*
Cell Cycle Proteins / metabolism
Cell Line
Cell Nucleus / metabolism*
Chromosomal Proteins, Non-Histone
DNA Replication
DNA, Complementary / chemistry
DNA, Complementary / genetics
DNA, Recombinant
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
Deoxyribonucleases / metabolism*
G2 Phase*
Gene Expression
HeLa Cells
Humans
Minichromosome Maintenance Proteins
Molecular Sequence Data
Origin Recognition Complex
Plasmids / genetics
Precipitin Tests
Protein Binding
RNA, Messenger / genetics
RNA, Messenger / metabolism
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae Proteins*
Schizosaccharomyces pombe Proteins*
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Two-Hybrid System Techniques

Substances

Cell Cycle Proteins
Chromosomal Proteins, Non-Histone
DNA, Complementary
DNA, Recombinant
DNA-Binding Proteins
MCM10 protein, S cerevisiae
MCM10 protein, human
ORC2 protein, human
Origin Recognition Complex
RNA, Messenger
Saccharomyces cerevisiae Proteins
Schizosaccharomyces pombe Proteins
cdc23 protein, S pombe
Deoxyribonucleases
Minichromosome Maintenance Proteins

Associated data

GENBANK/AB042719