Abstract
In an effort to identify potential upregulators of bcl-2 activity in t(14;18) follicular B lymphoma cells, we detected a hybrid bcl-2/IgH RNA transcribed in antisense orientation. This antisense transcript may contribute to upregulation of bcl-2 expression in t(14;18) cells, overlapping AU-rich motifs present in the 3'-untranslated region of bcl-2 mRNA. We have studied the enzymatic efficiency of a ribozyme directed towards the bcl-2 AU-rich region in a cell-free system determining its kinetic parameters.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3' Untranslated Regions / genetics*
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Base Pairing
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Burkitt Lymphoma / pathology
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Cell-Free System
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Chromosomes, Human, Pair 14 / genetics
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Chromosomes, Human, Pair 14 / ultrastructure
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Chromosomes, Human, Pair 18 / genetics
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Chromosomes, Human, Pair 18 / ultrastructure
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Gene Expression Regulation, Neoplastic*
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Genes, Immunoglobulin
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Humans
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Immunoglobulin Heavy Chains / genetics
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Kinetics
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Lymphoma, Follicular / genetics
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Lymphoma, Follicular / pathology
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Protein Structure, Tertiary
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Proto-Oncogene Proteins c-bcl-2 / chemistry
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Proto-Oncogene Proteins c-bcl-2 / physiology*
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RNA, Antisense / genetics*
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RNA, Catalytic / metabolism
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RNA, Messenger / genetics
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RNA, Neoplasm / genetics
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Substrate Specificity
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Translocation, Genetic / genetics
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Tumor Cells, Cultured
Substances
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3' Untranslated Regions
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Immunoglobulin Heavy Chains
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Proto-Oncogene Proteins c-bcl-2
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RNA, Antisense
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RNA, Catalytic
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RNA, Messenger
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RNA, Neoplasm
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hammerhead ribozyme RZ3-BL2