t(9;11)(p22;p15) in acute myeloid leukemia results in a fusion between NUP98 and the gene encoding transcriptional coactivators p52 and p75-lens epithelium-derived growth factor (LEDGF)

Cancer Res. 2000 Nov 15;60(22):6227-9.

Abstract

A t(9;11)(p22;p15) chromosomal translocation was identified in an adult patient with de novo acute myelogenous leukemia. Fluorescence in situ hybridization and Southern blot analysis mapped the 11p15 break-point to the NUP98 gene. Using 3' rapid amplification of cDNA ends, we have identified a chimeric mRNA that fused the NUP98 FXFG repeats in frame to the COOH-terminal portion of the gene encoding the transcriptional coactivators p52 and p75, also known as lens epithelium-derived growth factor (LEDGF). As expected, both NUP98-p52 and NUP98-p75 (LEDGF) chimeric mRNAs were detected by reverse transcription-PCR; however, the reciprocal p52/p75 (LEDGF)-NUP98 fusion mRNA was not detected. Our results demonstrate that this is the most 5' NUP98 fusion reported and reveal a previously unrecognized genetic target, the gene encoding p52/p75 (LEDGF).

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Artificial Gene Fusion
  • Base Sequence
  • Chromosomes, Human, Pair 11*
  • Chromosomes, Human, Pair 9*
  • Growth Substances / genetics*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Intercellular Signaling Peptides and Proteins*
  • Leukemia, Myeloid, Acute / genetics*
  • Male
  • Membrane Proteins / genetics
  • Middle Aged
  • Molecular Sequence Data
  • Nuclear Pore Complex Proteins*
  • Nuclear Proteins / genetics
  • RNA, Messenger / genetics
  • Recombinant Fusion Proteins / genetics
  • Repetitive Sequences, Nucleic Acid
  • Reverse Transcriptase Polymerase Chain Reaction
  • Trans-Activators / genetics*
  • Transcription Factors
  • Translocation, Genetic*

Substances

  • Adaptor Proteins, Signal Transducing
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Nuclear Pore Complex Proteins
  • Nuclear Proteins
  • PSIP1 protein, human
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • Trans-Activators
  • Transcription Factors
  • lens epithelium-derived growth factor
  • nuclear pore complex protein 98

Associated data

  • GENBANK/AF063020
  • GENBANK/AF098482
  • GENBANK/U41815