Abstract
A t(9;11)(p22;p15) chromosomal translocation was identified in an adult patient with de novo acute myelogenous leukemia. Fluorescence in situ hybridization and Southern blot analysis mapped the 11p15 break-point to the NUP98 gene. Using 3' rapid amplification of cDNA ends, we have identified a chimeric mRNA that fused the NUP98 FXFG repeats in frame to the COOH-terminal portion of the gene encoding the transcriptional coactivators p52 and p75, also known as lens epithelium-derived growth factor (LEDGF). As expected, both NUP98-p52 and NUP98-p75 (LEDGF) chimeric mRNAs were detected by reverse transcription-PCR; however, the reciprocal p52/p75 (LEDGF)-NUP98 fusion mRNA was not detected. Our results demonstrate that this is the most 5' NUP98 fusion reported and reveal a previously unrecognized genetic target, the gene encoding p52/p75 (LEDGF).
Publication types
-
Case Reports
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Adaptor Proteins, Signal Transducing
-
Amino Acid Sequence
-
Artificial Gene Fusion
-
Base Sequence
-
Chromosomes, Human, Pair 11*
-
Chromosomes, Human, Pair 9*
-
Growth Substances / genetics*
-
Humans
-
In Situ Hybridization, Fluorescence
-
Intercellular Signaling Peptides and Proteins*
-
Leukemia, Myeloid, Acute / genetics*
-
Male
-
Membrane Proteins / genetics
-
Middle Aged
-
Molecular Sequence Data
-
Nuclear Pore Complex Proteins*
-
Nuclear Proteins / genetics
-
RNA, Messenger / genetics
-
Recombinant Fusion Proteins / genetics
-
Repetitive Sequences, Nucleic Acid
-
Reverse Transcriptase Polymerase Chain Reaction
-
Trans-Activators / genetics*
-
Transcription Factors
-
Translocation, Genetic*
Substances
-
Adaptor Proteins, Signal Transducing
-
Growth Substances
-
Intercellular Signaling Peptides and Proteins
-
Membrane Proteins
-
Nuclear Pore Complex Proteins
-
Nuclear Proteins
-
PSIP1 protein, human
-
RNA, Messenger
-
Recombinant Fusion Proteins
-
Trans-Activators
-
Transcription Factors
-
lens epithelium-derived growth factor
-
nuclear pore complex protein 98
Associated data
-
GENBANK/AF063020
-
GENBANK/AF098482
-
GENBANK/U41815