Hunting the subset-specific genes of neuroblastoma: expression profiling and differential screening of the full-length-enriched oligo-capping cDNA libraries

M Ohira; T Shishikura; T Kawamoto; H Inuzuka; A Morohashi; H Takayasu; H Kageyama; N Takada; M Takahashi; S Sakiyama; Y Suzuki; S Sugano; H Kuma; I Nozawa; A Nakagawara

doi:10.1002/1096-911x(20001201)35:6<547::aid-mpo11>3.0.co;2-x

Hunting the subset-specific genes of neuroblastoma: expression profiling and differential screening of the full-length-enriched oligo-capping cDNA libraries

Med Pediatr Oncol. 2000 Dec;35(6):547-9. doi: 10.1002/1096-911x(20001201)35:6<547::aid-mpo11>3.0.co;2-x.

Authors

M Ohira¹, T Shishikura, T Kawamoto, H Inuzuka, A Morohashi, H Takayasu, H Kageyama, N Takada, M Takahashi, S Sakiyama, Y Suzuki, S Sugano, H Kuma, I Nozawa, A Nakagawara

Affiliation

¹ Division of Biochemistry, Chiba Cancer Center Research Institute, Chiba, Japan.

PMID: 11107114
DOI: 10.1002/1096-911x(20001201)35:6<547::aid-mpo11>3.0.co;2-x

Abstract

Background: Neuroblastoma (NBL) has a distinct nature in different prognostic subgroups.

Procedure: To understand the molecular mechanism of NBL's genesis and biology as well as that of the neural crest development, we constructed full-length-enriched cDNA libraries by an oligo-capping method from two different subsets of primary NBL, one with favorable biology and the other with MYCN amplification.

Results: Sequencing analysis of these libraries revealed that the expression profile was markedly different between both subsets. To identify the genes differentially expressed between the subsets, semi-quantitative RT-PCR analyses are proceeding.

Conclusion: So far, 54 transcripts have been found to be expressed at high levels in favorable NBLs, and significantly at low levels in unfavorable NBLs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression Regulation, Neoplastic / genetics*
Gene Library*
Humans
Mass Screening
Neuroblastoma / genetics*
Tumor Cells, Cultured