Abstract
To discern the T cell subtype associated with T cell differentiation, the expression of CD45RA and CD27 was measured from total CD8(high) cells and from human T cell lymphotropic virus type I (HTLV-I) Tax11-19 peptide-specific CD8(+) cells in peripheral blood lymphocytes of patients with HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Phenotypically defined memory and/or effector cells (CD45RA(-)CD27(+), CD45RA(+)CD27(-), and CD45RA(-)CD27(-)) were increased in HAM/TSP CD8(+) cells, compared with those of HTLV-I-seronegative healthy control subjects. The percentage of human leukocyte antigen (HLA)-DR-positive cells was also increased in CD8(+) cells of HAM/TSP, compared with those in HLA-DR(+)CD8(+) cells of healthy control subjects. HTLV-I provirus load correlated with the frequency of Tax11-19-specific CD8(+) cells. The high frequency of memory and/or effector type HTLV-I Tax11-19-specific CD8(+) cells suggests that continuous restimulation driven by HTLV-I antigens in vivo may be associated with the pathogenesis of HAM/TSP.
MeSH terms
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Adult
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CD8-Positive T-Lymphocytes / classification
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CD8-Positive T-Lymphocytes / immunology*
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Cytotoxicity Tests, Immunologic
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DNA, Viral / blood
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Female
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Flow Cytometry
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Gene Products, tax / chemistry
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Gene Products, tax / immunology*
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HLA-A2 Antigen / analysis
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Human T-lymphotropic virus 1 / genetics
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Human T-lymphotropic virus 1 / immunology
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Human T-lymphotropic virus 1 / isolation & purification
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Human T-lymphotropic virus 1 / physiology*
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Humans
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Immunoglobulin G / metabolism
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Immunologic Memory*
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Leukocyte Common Antigens / analysis
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Lymphocyte Activation
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Male
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Membrane Glycoproteins / metabolism
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Middle Aged
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Paraparesis, Tropical Spastic / immunology*
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Paraparesis, Tropical Spastic / physiopathology
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Paraparesis, Tropical Spastic / virology
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Peptides / chemical synthesis
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Peptides / chemistry
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Peptides / immunology
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Perforin
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Pore Forming Cytotoxic Proteins
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Proviruses
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T-Lymphocytes, Regulatory / immunology
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Tumor Necrosis Factor Receptor Superfamily, Member 7 / analysis
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Viral Load
Substances
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DNA, Viral
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Gene Products, tax
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HLA-A2 Antigen
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Immunoglobulin G
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Membrane Glycoproteins
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Peptides
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Pore Forming Cytotoxic Proteins
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Tumor Necrosis Factor Receptor Superfamily, Member 7
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Perforin
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Leukocyte Common Antigens