Abstract
Here we describe hemopoietic chimeras serving as a mouse model for NUP98-HOXA9-induced leukemia, which reproduced several of the phenotypes observed in human disease. Mice transplanted with bone marrow cells expressing NUP98-HOXA9 through retroviral transduction acquire a myeloproliferative disease (MPD) and eventually succumb to acute myeloid leukemia (AML). The NUP98 portion of the fusion protein was shown to be responsible for transforming a clinically silent pre-leukemic phase observed for Hoxa9 into a chronic, stem cell-derived MPD. The co-expression of NUP98-HOXA9 and Meis1 accelerated the transformation of MPD to AML, identifying a genetic interaction previously observed for Hoxa9 and Meis1. Our findings demonstrate the presence of overlapping yet distinct molecular mechanisms for MPD versus AML, illustrating the complexity of leukemic transformation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Bone Marrow Transplantation
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Cell Transformation, Neoplastic / genetics
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Chimera
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Gene Expression
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Hematopoietic Stem Cells / metabolism*
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Homeodomain Proteins / genetics
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Humans
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Leukemia, Experimental / etiology*
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Leukemia, Experimental / genetics
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / etiology
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics
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Leukemia, Myeloid, Acute / etiology
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Leukemia, Myeloid, Acute / genetics
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Mice
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Mice, Inbred C3H
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Mice, Inbred C57BL
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Myeloid Ecotropic Viral Integration Site 1 Protein
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Myeloproliferative Disorders / etiology
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Myeloproliferative Disorders / genetics
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Neoplasm Proteins / genetics
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Nuclear Pore Complex Proteins*
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Oncogene Proteins, Fusion / genetics*
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Retroviridae / genetics
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Transduction, Genetic
Substances
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Homeodomain Proteins
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MEIS1 protein, human
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Meis1 protein, mouse
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Myeloid Ecotropic Viral Integration Site 1 Protein
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NUP98-HOXA9 fusion protein, human
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Neoplasm Proteins
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Nuclear Pore Complex Proteins
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Oncogene Proteins, Fusion