Expression of tumor necrosis factor alpha and interleukin 1 beta in jejuna of volunteers after experimental challenge with Cryptosporidium parvum correlates with exposure but not with symptoms

Infect Immun. 2001 Feb;69(2):1172-4. doi: 10.1128/IAI.69.2.1172-1174.2001.

Abstract

Jejunal biopsies from volunteers challenged with Cryptosporidium parvum were examined for tumor necrosis factor alpha (TNF-alpha) and interleukin (IL)-1 beta mRNA. Postchallenge biopsies from 15 of 28 (54%) volunteers expressed TNF-alpha; 14% expressed IL-1 beta. Cytokine expression did not correlate with enteric symptoms, suggesting that TNF-alpha and IL-1 beta are not key mediators of diarrhea in human cryptosporidiosis.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Biopsy
  • Cryptosporidiosis / immunology*
  • Cryptosporidium parvum*
  • Diarrhea / etiology*
  • Humans
  • Interleukin-1 / biosynthesis*
  • Interleukin-1 / genetics
  • Interleukin-15 / biosynthesis
  • Jejunum / metabolism*
  • RNA, Messenger / analysis
  • Tumor Necrosis Factor-alpha / biosynthesis*
  • Tumor Necrosis Factor-alpha / genetics

Substances

  • Interleukin-1
  • Interleukin-15
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha