Inhibition of caspase-1 induces cell death in pancreatic carcinoma cells and potentially modulates expression levels of bcl-2 family proteins

S Schlosser; F Gansauge; M Ramadani; H G Beger; S Gansauge

doi:10.1016/s0014-5793(01)02144-5

Inhibition of caspase-1 induces cell death in pancreatic carcinoma cells and potentially modulates expression levels of bcl-2 family proteins

FEBS Lett. 2001 Feb 23;491(1-2):104-8. doi: 10.1016/s0014-5793(01)02144-5.

Authors

S Schlosser¹, F Gansauge, M Ramadani, H G Beger, S Gansauge

Affiliation

¹ Division of Molecular Oncology, Department of General Surgery, University of Ulm, Steinhövelstrasse 9, 89075, Ulm, Germany.

PMID: 11226429
DOI: 10.1016/s0014-5793(01)02144-5

Abstract

Caspase-1 (interleukin-1beta-converting enzyme) is reported to play an important role in the regulation of apoptosis. We investigated the inhibition of caspase-1 by the cell permeable caspase-1 inhibitor Ac-AAVALLPAVLLALLAP-YVAD.CHO in pancreatic carcinoma cells. Inhibition of caspase-1 induced a non-apoptotic/"necrotic-like" cell death in AsPC-1, BxPC-3, MiaPaCa-2 and Panc-1 cells. Expression levels of bcl-2 and bax were up-regulated in caspase-1 inhibitor-treated cells while that of bcl-x(L) remained unaltered. Our observations support our previous findings that caspase-1 is potentially involved in anti-apoptotic processes in pancreatic carcinoma.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis*
Blotting, Western
Caspase 1 / metabolism*
Caspase Inhibitors
Cell Cycle
DNA Fragmentation
Humans
Necrosis*
Oligopeptides / pharmacology
Pancreatic Neoplasms
Proto-Oncogene Proteins / metabolism
Proto-Oncogene Proteins c-bcl-2 / genetics
Proto-Oncogene Proteins c-bcl-2 / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
Up-Regulation
bcl-2-Associated X Protein

Substances

Ac-AAVALLPAVLLALLAPYVAD-CHO
BAX protein, human
Caspase Inhibitors
Oligopeptides
Proto-Oncogene Proteins
Proto-Oncogene Proteins c-bcl-2
bcl-2-Associated X Protein
Caspase 1