Abstract
We have established a panel of nine immortal cell lines from T cell malignancies which arose in mice transgenic for the SCL and LMO1 genes. Cells from the primary malignancies initially grew very slowly in vitro, loosely attached to a stromal layer, before gaining the ability to proliferate independently. Upon gaining the ability to proliferate in the absence of a stromal layer, these cell lines grew rapidly, doubling every 14-23 h, to a very high density, approaching 10(7) cells/ml. Whereas the tumors which arise in SCL/LMO1 double transgenic mice are typically diploid or pseudodiploid, the cell lines were all grossly aneuploid, suggesting the possibility that additional genetic events were selected for in vitro. Given that SCL and LMO1 gene activation are both commonly seen in human patients with T cell acute lymphoblastic leukemia, these cell lines may be a useful in vitro model for the human disease.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Basic Helix-Loop-Helix Transcription Factors
-
DNA-Binding Proteins / genetics*
-
Gene Expression Regulation, Neoplastic
-
Humans
-
LIM Domain Proteins
-
Leukemia, Experimental / genetics*
-
Leukemia, Experimental / pathology
-
Leukemia, T-Cell / genetics*
-
Leukemia, T-Cell / pathology
-
Metalloproteins / genetics*
-
Mice
-
Mice, Transgenic
-
Nuclear Proteins
-
Oncogene Proteins*
-
Proto-Oncogene Proteins*
-
T-Cell Acute Lymphocytic Leukemia Protein 1
-
Transcription Factors*
-
Transcriptional Activation
-
Tumor Cells, Cultured*
Substances
-
Basic Helix-Loop-Helix Transcription Factors
-
DNA-Binding Proteins
-
LIM Domain Proteins
-
LMO1 protein, human
-
Lmo1 protein, mouse
-
Metalloproteins
-
Nuclear Proteins
-
Oncogene Proteins
-
Proto-Oncogene Proteins
-
T-Cell Acute Lymphocytic Leukemia Protein 1
-
Tal1 protein, mouse
-
Transcription Factors
-
TAL1 protein, human