Enhanced expression of cyclin E and cyclin A in human hepatocellular carcinomas

R Ohashi; C Gao; M Miyazaki; K Hamazaki; T Tsuji; Y Inoue; T Uemura; R Hirai; N Shimizu; M Namba

Enhanced expression of cyclin E and cyclin A in human hepatocellular carcinomas

Anticancer Res. 2001 Jan-Feb;21(1B):657-62.

Authors

R Ohashi¹, C Gao, M Miyazaki, K Hamazaki, T Tsuji, Y Inoue, T Uemura, R Hirai, N Shimizu, M Namba

Affiliation

¹ Department of Cell Biology, Institute of Cellular and Molecular Biology, Okayama University Medical School, 2-5-1 Shikata, Okayama 700-8558, Japan.

PMID: 11299822

Abstract

Background: Disruption of the G1/S check point leads to uncontrolled cell growth, resulting in the development of cancers.

Materials and methods: Cell cycle regulatory molecules were investigated in 33 surgically resected hepatocellular carcinoma (HCC) samples from 30 patients by Western blotting.

Results: Enhanced expressions of cyclin E and cyclin A were detected at frequencies of 18/33 and 26/33 in HCCs, respectively, as compared with their neighboring noncancerous tissues. The enhanced expression of cyclin E, but not that of cyclin A, correlated with hyperphosphorylation of pRb and high frequency of Ki-67-positive cells. Thus, the HCCs with enhanced cyclin E expression probably contain a relatively large number of proliferating cancer cells.

Conclusions: The degree of cyclin E expression can be used as a prognostic parameter of HCC. In addition, cyclin E may become a molecular target in the treatment of HCCs.

MeSH terms

Aged
Aged, 80 and over
Blotting, Western
Carcinoma, Hepatocellular / chemistry*
Carcinoma, Hepatocellular / genetics
Carcinoma, Hepatocellular / metabolism
Cell Cycle Proteins*
Cyclin A / analysis*
Cyclin A / biosynthesis
Cyclin A / genetics
Cyclin E / analysis*
Cyclin E / biosynthesis
Cyclin E / genetics
Cyclin-Dependent Kinase Inhibitor p21
Cyclin-Dependent Kinase Inhibitor p27
Cyclins / analysis
Female
G1 Phase
Gene Expression Regulation, Neoplastic*
Genes, p53
Humans
Ki-67 Antigen / analysis
Liver Neoplasms / chemistry*
Liver Neoplasms / genetics
Liver Neoplasms / metabolism
Male
Microtubule-Associated Proteins / analysis
Middle Aged
Neoplasm Proteins / analysis*
Neoplasm Proteins / biosynthesis
Neoplasm Proteins / genetics
Phosphorylation
Protein Processing, Post-Translational
Retinoblastoma Protein / chemistry
Tumor Suppressor Proteins*

Substances

CDKN1A protein, human
Cell Cycle Proteins
Cyclin A
Cyclin E
Cyclin-Dependent Kinase Inhibitor p21
Cyclins
Ki-67 Antigen
Microtubule-Associated Proteins
Neoplasm Proteins
Retinoblastoma Protein
Tumor Suppressor Proteins
Cyclin-Dependent Kinase Inhibitor p27