Abstract
Apolipoprotein C-II (apoC-II), which is known to activate lipoprotein lipase (LPL), was identified by ordered differential display (ODD)-polymerase chain reaction (PCR) as a cDNA fragment exhibiting a distinct increase in expression during 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced differentiation of promonocytic U937 cells into monocytes and macrophages. The amount of apoC-II mRNA expression detectable in U937 cells significantly increased and reached a maximum 24-48 h after treatment with 32 nM TPA. apoC-II mRNA was also detected in monocytic THP-1 cells but was not detected in promyelocytic HL-60 cells. In healthy human tissues, the most significant expression of apoC-II mRNA was in the liver. Although apoC-II mRNA expression was markedly up-regulated during the induced differentiation of HL-60 cells into monocytes and macrophages with 32 nM TPA, such expression was not induced during the differentiation of HL-60 cells into granulocytes with 1.25% dimethyl sulfoxide. These results suggest that human apoC-II expression is induced at the transcription level during myelomonocytic differentiation and may confer an important role to macrophages involved in normal lipid metabolism and atherosclerosis.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells / drug effects
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3T3 Cells / metabolism
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Animals
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Apolipoprotein C-II
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Apolipoproteins C / biosynthesis*
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Apolipoproteins C / genetics
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Carcinoma / pathology
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Cell Differentiation
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Cyclin A / biosynthesis
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Cyclin A / genetics
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DNA, Complementary / genetics
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Dimethyl Sulfoxide / pharmacology
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Enzyme Activation
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Gene Expression Profiling
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Gene Expression Regulation, Leukemic / drug effects*
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Granulocytes / cytology
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Granulocytes / metabolism
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HL-60 Cells / cytology
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HL-60 Cells / drug effects*
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HL-60 Cells / metabolism
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Hematologic Neoplasms / pathology
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Humans
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Intestinal Mucosa / metabolism
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Jurkat Cells / drug effects
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Jurkat Cells / metabolism
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Kinesins / biosynthesis
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Kinesins / genetics
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Lipid Metabolism
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Lipoprotein Lipase / metabolism
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Liver / metabolism
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Macrophages / cytology
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Macrophages / metabolism
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Mice
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Monocytes / cytology
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Monocytes / metabolism*
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Myeloid Cells / drug effects
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Myeloid Cells / metabolism
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Neoplasm Proteins / biosynthesis*
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Neoplasm Proteins / genetics
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Polymerase Chain Reaction
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RNA, Messenger / biosynthesis
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Subtraction Technique
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Tetradecanoylphorbol Acetate / pharmacology
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Transcription, Genetic / drug effects
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Tumor Cells, Cultured / drug effects
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Tumor Cells, Cultured / metabolism
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U937 Cells / cytology
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U937 Cells / drug effects*
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U937 Cells / metabolism
Substances
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Apolipoprotein C-II
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Apolipoproteins C
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Cyclin A
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DNA, Complementary
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KIF2C protein, human
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Neoplasm Proteins
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RNA, Messenger
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Lipoprotein Lipase
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Kinesins
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Tetradecanoylphorbol Acetate
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Dimethyl Sulfoxide