Amplification of extracellular matrix and oncogenes in tat-transfected human salivary gland cell lines with expression of laminin, fibronectin, collagens I, III, IV, c-myc and p53

Arch Oral Biol. 2001 Jun;46(6):545-55. doi: 10.1016/s0003-9969(01)00014-0.

Abstract

Considerable progress has been made in the transfer of foreign genes into salivary glands in vivo using adenovirus vectors in rats. In an attempt to avoid the transient expression inherent, when using these vectors, retroviral vectors and human cell lines where used here in attempt to develop an in vitro model of HIV-associated salivary gland disease. The HIV-1-tat protein is increasingly implicated in the pathogenesis of the AIDS through altering the expression of strategic cellular genes. The purpose of this study was to transfect human salivary gland (HSG) cell lines in vitro, with the pHIV-1/LTR-tat plasmid, and examine the effect of tat on expression of matrix and basement membrane genes known to be important in the pathogenesis of salivary gland disease. HSG cells were transfected with HIV-1-tat plasmid by the lipofection method. Transfection was confirmed by polymerase chain reaction (PCR) and Southern blot, which verified that tat-specific DNA was present. Tat-mRNA was analysed by Northern blotting and quantified by reverse transcriptase polymerase chain reaction (RT-PCR) to demonstrate its expression. Numerous clones were found to contain integrated tat DNA sequences and analysis of mRNA showed stable expression of tat-specific RNA. Further analysis of mRNA expression for various marker proteins important in HIV pathogenesis showed that the HSG cell line transfected with HIV-1-tat, was associated with significant induction of mRNA expression for extracellular matrix protein. Tat-amplified transcription of the major basement membrane protein laminin, as well as of fibronectin, collagen I and III, and c-myc oncogene was demonstrated. Conversely, expression of p53 suppressor gene mRNA was reduced. Post-transfection expression of collagen IV was erratic and inconclusive. It was concluded that the presence of HIV-tat in this in vitro model of salivary ductal epithelial cell model alters the mRNA expression of several matrix, basement membrane and oncoproteins known to be involved in HIV pathogenesis. These cell lines provide a useful system for studying the role of tat in the immunopathogenesis of HIV-associated salivary gland disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Basement Membrane / metabolism
  • Blotting, Southern
  • Cell Line
  • Collagen / genetics*
  • DNA, Viral / analysis
  • Epithelial Cells / metabolism
  • Extracellular Matrix / metabolism
  • Extracellular Matrix Proteins / genetics*
  • Fibronectins / genetics*
  • Gene Amplification*
  • Gene Expression Regulation, Viral
  • Genes, myc / genetics*
  • Genes, p53 / genetics*
  • Genes, tat / genetics*
  • HIV / genetics*
  • HIV Infections / genetics
  • Humans
  • Laminin / genetics*
  • Oncogenes / genetics*
  • Plasmids
  • RNA, Messenger / analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Salivary Ducts / metabolism
  • Salivary Gland Diseases / genetics
  • Salivary Gland Diseases / virology
  • Salivary Glands / metabolism*
  • Transcription, Genetic
  • Transfection*

Substances

  • DNA, Viral
  • Extracellular Matrix Proteins
  • Fibronectins
  • Laminin
  • RNA, Messenger
  • Collagen