Characterization of human immunodeficiency virus type 1 in saliva and blood plasma by V3-specific heteroduplex tracking assay and genotype analyses

J Virol. 2001 May;75(10):4936-40. doi: 10.1128/JVI.75.10.4936-4940.2001.

Abstract

The gp120 V3-encoding region of human immunodeficiency virus type 1 (HIV-1) RNA derived from the saliva and blood plasma of 11 individuals was characterized by heteroduplex tracking assay and sequence analyses. R5-like viral variants were identified in both fluids of all subjects. X4-like variants were detected in the plasma and/or saliva of three subjects, indicating that X4-like variants are not excluded from the saliva compartment. Viral subpopulations were similar in both fluids of most subjects, suggesting that HIV-1 in oral fluids and blood may stem from a common source. These findings raise the possibility of using saliva as a noninvasive fluid for evaluating and monitoring viral evolution in infected persons.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA, Viral
  • Genotype
  • HIV Envelope Protein gp120 / genetics*
  • HIV Infections / virology*
  • HIV-1 / classification
  • HIV-1 / genetics*
  • Heteroduplex Analysis
  • Humans
  • Molecular Sequence Data
  • Peptide Fragments / genetics*
  • Plasma / virology
  • Saliva / virology

Substances

  • DNA, Viral
  • HIV Envelope Protein gp120
  • HIV envelope protein gp120 (305-321)
  • Peptide Fragments

Associated data

  • GENBANK/AF362846
  • GENBANK/AF362847
  • GENBANK/AF362848
  • GENBANK/AF362849
  • GENBANK/AF362850
  • GENBANK/AF362851
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  • GENBANK/AF362853
  • GENBANK/AF362854
  • GENBANK/AF362855
  • GENBANK/AF362856
  • GENBANK/AF362857
  • GENBANK/AF362858
  • GENBANK/AF362859
  • GENBANK/AF362860
  • GENBANK/AF362861
  • GENBANK/AF362862
  • GENBANK/AF362863
  • GENBANK/AF362864
  • GENBANK/AF362865
  • GENBANK/AF362866
  • GENBANK/AF362867
  • GENBANK/AF362868
  • GENBANK/AF362869
  • GENBANK/AF362870
  • GENBANK/AF362871
  • GENBANK/AF362872
  • GENBANK/AF362873
  • GENBANK/AF362874
  • GENBANK/AF362875
  • GENBANK/AF362876
  • GENBANK/AF362877
  • GENBANK/AF362878
  • GENBANK/AF362879
  • GENBANK/AF362880
  • GENBANK/AF362881
  • GENBANK/AF362882
  • GENBANK/AF362883
  • GENBANK/AF362884
  • GENBANK/AF362885