Abstract
We examined IL-10 and IL-10 receptor (IL-10R) gene expression in 44 xenografts of human non-small cell lung cancer (NSCLC) by reverse transcription-polymerase chain reaction (RT-PCR) assay with human-specific primers. IL-10 gene expression was detected in 10 (22.7%) of 44 xenografts (0/11 adenocarcinomas, 6/19 squamous cell carcinomas, 4/14 large cell carcinomas) and IL-10R expression in 8 (18.2%) xenografts. The IL-10-positive xenografts immunohistochemically showed significantly increased growth fractions of neoplastic cells (471.0 +/- 112.2) relative to IL-10-negative xenografts (208.4 +/- 96.3) (p=0.009, Mann-Whitney U test), and showed significantly increased vascular number (p=0.008, Mann-Whitney U test). These results suggested that IL-10 expression is correlated with growth features in human NSCLC.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Carcinoma, Non-Small-Cell Lung / metabolism
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Carcinoma, Non-Small-Cell Lung / pathology*
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DNA Primers / chemistry
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Gene Expression
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Growth Substances / biosynthesis
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Growth Substances / genetics
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Humans
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Immunoenzyme Techniques
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Interleukin-10 / biosynthesis
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Interleukin-10 / genetics*
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Lung Neoplasms / metabolism
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Lung Neoplasms / pathology*
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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Neoplasm Transplantation
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Neovascularization, Pathologic / pathology
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RNA, Messenger / metabolism
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Receptors, Interleukin / biosynthesis
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Receptors, Interleukin / genetics
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Receptors, Interleukin-10
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Reverse Transcriptase Polymerase Chain Reaction
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Transplantation, Heterologous
Substances
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DNA Primers
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Growth Substances
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RNA, Messenger
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Receptors, Interleukin
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Receptors, Interleukin-10
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Interleukin-10