Abstract
We investigated possible expression and function in mast cells of protein kinase C (PKC) theta, a member of the PKC family with demonstrated function in a limited range of cell types. We found that PKC theta is expressed in bone marrow-derived mast cells and in the RBL-2H3 mast cell line. PKC theta underwent translocation to the membrane in response to Fcepsilon receptor I (FcepsilonR I) activation. Receptor activation induced phosphorylation of PKC theta. The tyrosine phosphorylation of PKC theta is delayed relative to PKC delta and coincides temporally with PKC theta association with c-src family members Lyn and SRC: Studies of RBL-2H3 cells transduced with PKC theta constructs indicated a role for PKC theta in receptor-induced activation of extracellular regulated kinases, interleukin-3 gene transcription, and degranulation in response to antigen stimulation. These studies extend the known functions of PKCtheta to another important immune cell type and indicate the concurrent participation of multiple PKCs in the FcepsilonR I-mediated response of mast cells.
MeSH terms
-
Animals
-
Antigens / immunology
-
Antigens / pharmacology
-
Cell Degranulation
-
Cell Line
-
Cells, Cultured
-
Cross-Linking Reagents
-
Dinitrophenols / immunology
-
Dinitrophenols / pharmacology
-
Enzyme Activation
-
Gene Expression
-
Humans
-
Interleukin-3 / genetics
-
Isoenzymes / genetics
-
Isoenzymes / immunology*
-
Isoenzymes / metabolism
-
Leukocytes, Mononuclear / drug effects
-
Leukocytes, Mononuclear / immunology
-
Mast Cells / cytology
-
Mast Cells / drug effects
-
Mast Cells / immunology*
-
Mice
-
Mitogen-Activated Protein Kinase 1 / metabolism
-
Mitogen-Activated Protein Kinase 3
-
Mitogen-Activated Protein Kinases / metabolism
-
Phosphorylation
-
Protein Kinase C / genetics
-
Protein Kinase C / immunology*
-
Protein Kinase C / metabolism
-
Protein Kinase C-theta
-
RNA, Messenger
-
Receptors, IgE / immunology*
-
Serum Albumin / immunology
-
Serum Albumin / pharmacology
-
Signal Transduction / immunology*
-
src-Family Kinases / metabolism
Substances
-
Antigens
-
Cross-Linking Reagents
-
Dinitrophenols
-
Interleukin-3
-
Isoenzymes
-
RNA, Messenger
-
Receptors, IgE
-
Serum Albumin
-
dinitrophenyl-human serum albumin conjugate
-
lyn protein-tyrosine kinase
-
src-Family Kinases
-
PRKCQ protein, human
-
Prkcq protein, mouse
-
Protein Kinase C
-
Protein Kinase C-theta
-
Mitogen-Activated Protein Kinase 1
-
Mitogen-Activated Protein Kinase 3
-
Mitogen-Activated Protein Kinases