Fluoropyrimidine sensitivity of human MCF-7 breast cancer cells stably transfected with human uridine phosphorylase

Br J Cancer. 2001 Jun 15;84(12):1677-80. doi: 10.1054/bjoc.2001.1833.

Abstract

The relationship between uridine phosphorylase (UP) expression level in cancer cells and the tumour sensitivity to fluoropyrimidines is unclear. In this study, we found that UP overexpression by gene transfer, and the subsequent efficient metabolic activation of 5-fluorouracil (5-FU) by the ribonucleotide pathway, does not increase the fluoropyrimidine sensitivity of MCF-7 human cancer cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antimetabolites, Antineoplastic / pharmacology*
  • Breast Neoplasms / pathology*
  • Female
  • Fluorouracil / pharmacology*
  • Genetic Therapy
  • Humans
  • Ribonucleotides
  • Transfection*
  • Tumor Cells, Cultured
  • Uridine Phosphorylase / biosynthesis*
  • Uridine Phosphorylase / genetics
  • Uridine Phosphorylase / metabolism

Substances

  • Antimetabolites, Antineoplastic
  • Ribonucleotides
  • Uridine Phosphorylase
  • Fluorouracil