Leptin receptor long-form signalling in a human liver cell line

Cytokine. 2001 May 21;14(4):225-9. doi: 10.1006/cyto.2001.0871.

Abstract

Expression of the long form of the leptin receptor was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting in the human liver cell line WRL68. Leptin (50-200 nM) significantly increased tyrosine phosphorylation of STAT cytoplasmic transcription factors STAT3 and STAT5b in a dose-dependent manner and produced a gel-shift with STAT3- and STAT5-specific oligonucleotides. WRL68 cells therefore provide the first human in vitro hepatocyte system in which to study leptin receptor-mediated signalling and to elucidate the role of leptin in liver.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carrier Proteins / biosynthesis
  • Carrier Proteins / physiology*
  • Cell Line
  • DNA-Binding Proteins / metabolism
  • Growth Substances / pharmacology
  • Humans
  • Leptin / metabolism
  • Leptin / pharmacology
  • Liver / cytology
  • Liver / metabolism
  • Liver / physiology*
  • Milk Proteins*
  • Phosphorylation
  • Receptors, Cell Surface*
  • Receptors, Cytokine / biosynthesis
  • Receptors, Cytokine / physiology*
  • Receptors, Leptin
  • STAT3 Transcription Factor
  • STAT5 Transcription Factor
  • Signal Transduction / physiology*
  • Trans-Activators / metabolism
  • Tyrosine / metabolism

Substances

  • Carrier Proteins
  • DNA-Binding Proteins
  • Growth Substances
  • LEPR protein, human
  • Leptin
  • Milk Proteins
  • Receptors, Cell Surface
  • Receptors, Cytokine
  • Receptors, Leptin
  • STAT3 Transcription Factor
  • STAT3 protein, human
  • STAT5 Transcription Factor
  • STAT5B protein, human
  • Trans-Activators
  • Tyrosine