Abstract
Expression of the long form of the leptin receptor was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting in the human liver cell line WRL68. Leptin (50-200 nM) significantly increased tyrosine phosphorylation of STAT cytoplasmic transcription factors STAT3 and STAT5b in a dose-dependent manner and produced a gel-shift with STAT3- and STAT5-specific oligonucleotides. WRL68 cells therefore provide the first human in vitro hepatocyte system in which to study leptin receptor-mediated signalling and to elucidate the role of leptin in liver.
Copyright 2001 Academic Press.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Carrier Proteins / biosynthesis
-
Carrier Proteins / physiology*
-
Cell Line
-
DNA-Binding Proteins / metabolism
-
Growth Substances / pharmacology
-
Humans
-
Leptin / metabolism
-
Leptin / pharmacology
-
Liver / cytology
-
Liver / metabolism
-
Liver / physiology*
-
Milk Proteins*
-
Phosphorylation
-
Receptors, Cell Surface*
-
Receptors, Cytokine / biosynthesis
-
Receptors, Cytokine / physiology*
-
Receptors, Leptin
-
STAT3 Transcription Factor
-
STAT5 Transcription Factor
-
Signal Transduction / physiology*
-
Trans-Activators / metabolism
-
Tyrosine / metabolism
Substances
-
Carrier Proteins
-
DNA-Binding Proteins
-
Growth Substances
-
LEPR protein, human
-
Leptin
-
Milk Proteins
-
Receptors, Cell Surface
-
Receptors, Cytokine
-
Receptors, Leptin
-
STAT3 Transcription Factor
-
STAT3 protein, human
-
STAT5 Transcription Factor
-
STAT5B protein, human
-
Trans-Activators
-
Tyrosine