Abstract
A yeast three-hybrid system was employed to analyze interactions in vivo between H1 RNA, the RNA subunit of human nuclear RNase P, and eight of the protein subunits of the enzyme. The genetic analysis indicates that subunits Rpp21, Rpp29, Rpp30, and Rpp38 interact directly with H1 RNA. The results of direct UV crosslinking studies of the purified RNase P holoenzyme confirm the results of the three-hybrid assay.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Blotting, Western
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Cell Nucleus / enzymology*
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Endoribonucleases / chemistry
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Endoribonucleases / metabolism*
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HeLa Cells
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Humans
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RNA / metabolism*
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RNA, Catalytic / chemistry
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RNA, Catalytic / metabolism*
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RNA-Binding Proteins / metabolism*
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Ribonuclease P
Substances
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RNA, Catalytic
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RNA-Binding Proteins
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RNA
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Endoribonucleases
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RPP14 protein, human
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Ribonuclease P