Abstract
The transcription factor nuclear factor kappa B (NF-kappa B) plays a pivotal role in inflammatory processes through induction of adhesion molecules and chemokines. The zinc finger molecule A20 is an important negative regulator of NF-kappa B. The mechanism utilized by A20 is not fully understood, but A20 has been shown to bind to tumour-necrosis-factor-receptor-associated factor (TRAF) molecules, which are necessary for pro-inflammatory cytokine signalling. We report two novel genes, Cezanne (cellular zinc finger anti-NF-kappa B) and TRABID (TRAF-binding domain), with sequence similarity to A20. Co-immunoprecipitation studies indicated that TRAF6 was able to interact with both Cezanne and TRABID. In contrast, reporter gene experiments revealed a specific ability of Cezanne to down-regulate NF-kappa B. It is likely, therefore, that Cezanne participates in the regulation of inflammatory processes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Cells, Cultured
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Cloning, Molecular
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Cysteine Endopeptidases
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DNA-Binding Proteins
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Endopeptidases / genetics
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Endopeptidases / isolation & purification*
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Endopeptidases / metabolism
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Gene Expression Regulation
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Humans
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Intracellular Signaling Peptides and Proteins
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Mice
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Molecular Sequence Data
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NF-kappa B / metabolism*
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Nuclear Proteins
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Proteins / genetics
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Proteins / isolation & purification*
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Proteins / metabolism
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Sequence Homology, Amino Acid
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Subcellular Fractions
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TNF Receptor-Associated Factor 6
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Transcription, Genetic
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Tumor Necrosis Factor alpha-Induced Protein 3
Substances
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DNA-Binding Proteins
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Intracellular Signaling Peptides and Proteins
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NF-kappa B
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Nuclear Proteins
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Proteins
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TNF Receptor-Associated Factor 6
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Endopeptidases
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ZRANB1 protein, human
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TNFAIP3 protein, human
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Tumor Necrosis Factor alpha-Induced Protein 3
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Cysteine Endopeptidases
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Tnfaip3 protein, mouse
Associated data
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GENBANK/AJ252060
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GENBANK/AJ293573