Abstract
The human snRNA promoters contain a proximal sequence element (PSE) required for basal transcription and a distal sequence element (DSE) required for activated transcription. The PSE recruits the multisubunit factor SNAPc, whereas the DSE recruits Oct-1. Oct-1 and SNAPc bind cooperatively to DNA when their respective binding sites are moved into proximity through a mechanism that involves a defined protein-protein contact. Here, we show that on the natural U6 promoter, cooperative binding of Oct-1 and SNAPc is mediated by a positioned nucleosome that resides between the DSE and the PSE. This cooperative binding requires the same protein-protein contact as cooperative binding to closely spaced sites on naked DNA and mediates transcription activation.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Allosteric Site
-
DNA / chemistry
-
DNA / genetics
-
DNA / metabolism
-
DNA Footprinting
-
DNA-Binding Proteins / chemistry*
-
DNA-Binding Proteins / metabolism*
-
HeLa Cells
-
Host Cell Factor C1
-
Humans
-
Micrococcal Nuclease / metabolism
-
Models, Biological
-
Molecular Conformation
-
Nucleosomes / chemistry
-
Nucleosomes / genetics
-
Nucleosomes / metabolism*
-
Octamer Transcription Factor-1
-
Promoter Regions, Genetic / genetics*
-
Protein Binding
-
Protein Structure, Tertiary
-
RNA, Messenger / genetics
-
RNA, Messenger / metabolism
-
RNA, Small Nuclear / genetics*
-
Response Elements / genetics
-
Templates, Genetic
-
Transcription Factors / chemistry*
-
Transcription Factors / metabolism*
-
Transcriptional Activation
Substances
-
DNA-Binding Proteins
-
HCFC1 protein, human
-
Host Cell Factor C1
-
Nucleosomes
-
Octamer Transcription Factor-1
-
POU2F1 protein, human
-
RNA, Messenger
-
RNA, Small Nuclear
-
SNAPC protein complex, human
-
Transcription Factors
-
U6 small nuclear RNA
-
DNA
-
Micrococcal Nuclease