Objective: To investigate the relationship between methylenetetrahydrofolate reductase(MTHFR) gene polymorphism and diabetic nephropathy(DN).
Methods: The technology of polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) was used to detect MTHFR gene C677T mutation in 85 healthy controls, 79 type-2 diabetics without DN, and 82 type-2 diabetics with DN confirmed by urine microalbuminous detection.
Results: The frequencies of MTHFR gene variant genotypes and alleles in the patients with DN patients were significantly higher than those in the patients without DN and those in healthy controls (P<0.01).
Conclusion: MTHFR gene C677T mutation is probably one of the genetic risk factors for diabetic nephropathy in Chinese Hans.