Abstract
Inducible cAMP early repressor (ICER) has been shown to be an important mediator of cAMP antiproliferative activity. In this report, it was found that cAMP retards LNCaP cell growth; in contrast, cAMP inhibits the growth of PC-3 and DU-145 cells. ICER protein levels were markedly reduced in prostate cancer epithelial cells and undetectable and uninducible by cAMP in LNCaP and DU 145 cells. Forced expression of ICER in LNCaP cells caused inhibition of cell growth and thymidine incorporation and halted cells at the G(1) phase of the cell cycle. These ICER-bearing LNCaP cells were rendered unable to grow in soft agar and unable to form tumors in nude mice. These results suggest that deregulation of ICER expression may be related to carcinogenesis of the prostate gland.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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8-Bromo Cyclic Adenosine Monophosphate / pharmacology
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Cell Division / drug effects
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Cell Division / physiology
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Cell Line, Transformed
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Cell Transformation, Neoplastic / genetics
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Cell Transformation, Neoplastic / metabolism
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Cyclic AMP / physiology*
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Cyclic AMP Response Element Modulator
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DNA-Binding Proteins / biosynthesis*
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DNA-Binding Proteins / genetics
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Gene Expression Regulation, Neoplastic / physiology
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Genes, Tumor Suppressor
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Humans
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Male
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Prostatic Neoplasms / genetics
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Prostatic Neoplasms / metabolism*
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Prostatic Neoplasms / pathology*
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Repressor Proteins / biosynthesis*
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Repressor Proteins / genetics
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Signal Transduction / physiology
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Transfection
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Tumor Cells, Cultured
Substances
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DNA-Binding Proteins
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Repressor Proteins
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Cyclic AMP Response Element Modulator
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8-Bromo Cyclic Adenosine Monophosphate
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Cyclic AMP