Characterization of human glucose transporter (GLUT) 11 (encoded by SLC2A11), a novel sugar-transport facilitator specifically expressed in heart and skeletal muscle

Biochem J. 2001 Oct 15;359(Pt 2):443-9. doi: 10.1042/0264-6021:3590443.

Abstract

Human GLUT11 (encoded by the solute carrier 2A11 gene, SLC2A11) is a novel sugar transporter which exhibits significant sequence similarity with the members of the GLUT family. The amino acid sequence deduced from its cDNAs predicts 12 putative membrane-spanning helices and all the motifs (sugar-transporter signatures) that have previously been shown to be essential for sugar-transport activity. The closest relative of GLUT11 is the fructose transporter GLUT5 (sharing 41.7% amino acid identity with GLUT11). The human GLUT11 gene (SLC2A11) consists of 12 exons and is located on chromosome 22q11.2. In human tissues, a 7.2 kb transcript of GLUT11 was detected exclusively in heart and skeletal muscle. Transfection of COS-7 cells with GLUT11 cDNA significantly increased the glucose-transport activity reconstituted from membrane extracts as well as the specific binding of the sugar-transporter ligand cytochalasin B. In contrast to that of GLUT4, the glucose-transport activity of GLUT11 was markedly inhibited by fructose. It is concluded that GLUT11 is a novel, muscle-specific transport facilitator that is a member of the extended GLUT family of sugar/polyol-transport facilitators.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • COS Cells
  • Cloning, Molecular
  • Cytochalasin B / metabolism
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • Exons
  • Gene Expression
  • Genome, Human
  • Glucose Transport Proteins, Facilitative
  • Humans
  • In Vitro Techniques
  • Models, Molecular
  • Molecular Sequence Data
  • Monosaccharide Transport Proteins / chemistry
  • Monosaccharide Transport Proteins / classification
  • Monosaccharide Transport Proteins / genetics*
  • Monosaccharide Transport Proteins / metabolism*
  • Muscle, Skeletal / metabolism*
  • Myocardium / metabolism*
  • Rats
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Transfection

Substances

  • DNA, Complementary
  • Glucose Transport Proteins, Facilitative
  • Monosaccharide Transport Proteins
  • Recombinant Proteins
  • SLC2A11 protein, human
  • Cytochalasin B

Associated data

  • GENBANK/AJ271290