Abstract
MSH2 and MLH1 are proteins involved in DNA reparation. They are mutated in some forms of colon cancer, i.e. hereditary non-polyposis carcinomas and a subset of sporadic carcinomas. We have studied the expression of MSH2 and MLH1 in a retrospective series of 225 colorectal carcinomas by immunohistochemistry. The results were compared to molecular tests of microsatellite instability using amplification by PCR of BAT-25 and BAT-26 repeated sequences and to histoclinical data. Positivity of both proteins was never associated with MSI phenotype. MSH2 and/or MLH1 negative tumors were frequently tumors of the proximal colon; in this subpopulation or proximal tumors, MSH2 and/or MLH1 negativity was associated with a longer disease-free survival.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing
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Aged
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Base Pair Mismatch*
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Carrier Proteins
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Colonic Neoplasms / chemistry*
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Colonic Neoplasms / diagnosis*
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DNA Repair
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DNA, Neoplasm / analysis
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DNA-Binding Proteins*
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Disease-Free Survival
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Female
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Humans
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Immunoenzyme Techniques
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Male
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Microsatellite Repeats
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MutL Protein Homolog 1
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MutS Homolog 2 Protein
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Neoplasm Proteins / analysis*
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Neoplasm Proteins / genetics
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Nuclear Proteins
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Polymerase Chain Reaction
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Prognosis
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Proto-Oncogene Proteins / analysis*
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Proto-Oncogene Proteins / genetics
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Retrospective Studies
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Survival Analysis
Substances
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Adaptor Proteins, Signal Transducing
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Carrier Proteins
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DNA, Neoplasm
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DNA-Binding Proteins
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MLH1 protein, human
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Neoplasm Proteins
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Nuclear Proteins
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Proto-Oncogene Proteins
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MSH2 protein, human
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MutL Protein Homolog 1
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MutS Homolog 2 Protein