The human ABCG4 gene is regulated by oxysterols and retinoids in monocyte-derived macrophages

Biochem Biophys Res Commun. 2001 Oct 26;288(2):483-8. doi: 10.1006/bbrc.2001.5756.

Abstract

Here we report the induction of gene expression of ABCG4, a member of the ABC transporter subfamily G, from human macrophages by oxysterols and retinoids, agonists of the nuclear receptors LXR and RXR. The cloned ABCG4 transcript has a size of 3.5 kb and contains an open reading frame which encodes a polypeptide of 646 amino acids. Structurally, the putative ABC transporter protein consists of a nucleotide binding fold followed by a cluster of six transmembrane-spanning domains and thus conforms to the group of half-size ABC transporters. Among the human ABC transporter subfamily G members the novel transporter shows highest protein sequence homology and identity to ABCG1 (84 and 72%, respectively). Analysis of the genomic organization demonstrates that the ABCG4 gene is composed of at least 14 exons which extend across a region of 12.6 kb in size on chromosome 11q23.3. Based on its structural features and an LXR/RXR-responsive regulation similar to the cellular lipid export protein ABCA1, we conclude that ABCG4 may be involved in macrophage lipid homeostasis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily G
  • ATP Binding Cassette Transporter, Subfamily G, Member 1
  • ATP-Binding Cassette Transporters / chemistry
  • ATP-Binding Cassette Transporters / genetics*
  • Amino Acid Sequence
  • Animals
  • Chromosome Mapping
  • Chromosomes, Human, Pair 11
  • Cloning, Molecular
  • DNA, Complementary / analysis
  • Exons
  • Gene Expression Regulation / drug effects*
  • Genome, Human
  • Humans
  • Introns
  • Macrophages / drug effects*
  • Macrophages / physiology
  • Mice
  • Molecular Sequence Data
  • Monocytes / physiology
  • Retinoids / pharmacology*
  • Sequence Homology, Amino Acid

Substances

  • ABCG1 protein, human
  • ABCG4 protein, human
  • ATP Binding Cassette Transporter, Subfamily G
  • ATP Binding Cassette Transporter, Subfamily G, Member 1
  • ATP-Binding Cassette Transporters
  • Abcg4 protein, mouse
  • DNA, Complementary
  • Retinoids

Associated data

  • GENBANK/AJ308237
  • GENBANK/AJ308238
  • GENBANK/AJ308239
  • GENBANK/AJ308240
  • GENBANK/AJ308241
  • GENBANK/AJ308242
  • GENBANK/AJ308243
  • GENBANK/AJ308244
  • GENBANK/AJ308245
  • GENBANK/AJ308246
  • GENBANK/AJ308247
  • GENBANK/AJ308248
  • GENBANK/AJ308249
  • GENBANK/AJ308250
  • GENBANK/AJ308251