Downmodulation of bFGF-binding protein expression following restoration of p53 function

Cancer Gene Ther. 2001 Oct;8(10):771-82. doi: 10.1038/sj.cgt.7700361.

Abstract

Angiogenesis is a requirement for solid tumor growth. Therefore, inhibition of this neovascularization is one mechanism by which restoration of wtp53 function may lead to tumor regression. Here we report that adenoviral vector-mediated wild-type p53 transduction results in growth inhibition of squamous cell carcinoma of the head and neck tumor cells both in vitro and in a xenograft mouse model. This growth inhibition is associated with the down-regulation of the expression of fibroblast growth factor binding protein, a secreted protein required for the activation of angiogenic factor basic FGF. These findings suggest that wtp53-induced tumor regression is due, at least in part, to antiangiogenesis mediated by the downmodulation of fibroblast growth factor binding protein.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviridae
  • Animals
  • Blotting, Northern
  • Carcinoma, Squamous Cell / genetics
  • Carcinoma, Squamous Cell / metabolism*
  • Carcinoma, Squamous Cell / pathology
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Division / genetics
  • Collagen / chemistry
  • DNA Primers / chemistry
  • Down-Regulation / physiology*
  • Drug Combinations
  • Head and Neck Neoplasms / genetics
  • Head and Neck Neoplasms / metabolism*
  • Head and Neck Neoplasms / pathology
  • Humans
  • Intercellular Signaling Peptides and Proteins
  • Intracellular Signaling Peptides and Proteins
  • Laminin / chemistry
  • Mice
  • Mice, Nude
  • Neoplasm Transplantation
  • Neovascularization, Pathologic / pathology
  • Proteoglycans / chemistry
  • RNA / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Cells, Cultured
  • Tumor Suppressor Protein p53 / genetics
  • Tumor Suppressor Protein p53 / metabolism*

Substances

  • Carrier Proteins
  • DNA Primers
  • Drug Combinations
  • Fgfbp1 protein, mouse
  • Intercellular Signaling Peptides and Proteins
  • Intracellular Signaling Peptides and Proteins
  • Laminin
  • Proteoglycans
  • Tumor Suppressor Protein p53
  • matrigel
  • FGFBP1 protein, human
  • RNA
  • Collagen