Abstract
In CD4+ UE160 cells with inducible expression of gp160, mechanisms of apoptosis induced by human immunodeficiency virus (HIV) Env protein were analyzed. Induction of gp160 caused intracellular calcium increase followed by the release of cytochrome c from mitochondria, which was inhibited by calcineurin inhibitors. Association of BAD with Bcl-xL was observed, and a portion of BAD was dephosphorylated after induction of gp160. These data suggested that calcineurin plays a role in the HIV Env-induced apoptosis in a mitochondrion-dependent way.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis*
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CD4-Positive T-Lymphocytes / drug effects*
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CD4-Positive T-Lymphocytes / metabolism
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CD4-Positive T-Lymphocytes / pathology
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Cadmium Chloride / pharmacology
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Calcineurin / metabolism*
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Calcium / pharmacology*
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HIV Envelope Protein gp160 / genetics
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HIV Envelope Protein gp160 / metabolism
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Humans
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Mitochondria / drug effects*
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Mitochondria / metabolism
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Mitochondria / pathology
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Transfection
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U937 Cells
Substances
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HIV Envelope Protein gp160
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Calcineurin
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Cadmium Chloride
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Calcium