Abstract
To explore the antiproliferative effects of rhumAbHER2 on head and neck squamous carcinoma cell (HNSCC) lines and breast cancer cell lines (BCCLs) and to evaluate the combined effects with irradiation, 2 human HNSCC lines and 2 BCCLs were exposed to rhumAbHER2 with or without irradiation. The results showed that combined treatment enhanced the growth and colonization inhibitory effects of rhumAbHER2 or irradiation. Interestingly, the apoptotic cell fraction produced by irradiation disappeared on combined treatment. This disappearance was associated with repression of p53 and Bax upregulation induced by irradiation, but conservation of the upregulation of p27. Based on these results, rhumAbHER2 and irradiation may be a new strategy for treating HNSCC and breast cancers. In addition, the upregulation of cyclin-dependent kinase inhibitors by rhumAbHER2 may occur upstream of irradiation-induced p53 upregulation.
Copyright 2001 Wiley-Liss, Inc.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis
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Blotting, Western
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Breast Neoplasms / radiotherapy
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Breast Neoplasms / therapy
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Carcinoma, Squamous Cell / radiotherapy
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Carcinoma, Squamous Cell / therapy
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Cell Cycle
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Cell Division / drug effects
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Cloning, Molecular
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Combined Modality Therapy
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Dose-Response Relationship, Drug
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Dose-Response Relationship, Radiation
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Flow Cytometry
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Genes, erbB-2 / genetics
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Genes, p53 / genetics
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Head and Neck Neoplasms / radiotherapy
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Head and Neck Neoplasms / therapy*
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Humans
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Immunotherapy / methods*
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In Situ Nick-End Labeling
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Proto-Oncogene Proteins / biosynthesis
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Proto-Oncogene Proteins c-bcl-2*
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Receptor, ErbB-2 / immunology*
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Time Factors
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Tumor Cells, Cultured
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Tumor Suppressor Protein p53 / biosynthesis
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Up-Regulation
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bcl-2-Associated X Protein
Substances
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BAX protein, human
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-bcl-2
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Tumor Suppressor Protein p53
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bcl-2-Associated X Protein
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Receptor, ErbB-2