Abstract
Aim:
To investigate role of apoptosis related genes Fas/FasL in azoospermia or oligozoospermia induced by testosterone undecanoate.
Methods:
TUNEL was used to detect the apoptotic signal of testicular cells. Immunohistochemistry and Western blot were used to quantitatively or qualitatively analyze the expression of these apoptosis-related proteins.
Results:
After injection of testosterone undecanoate, both the apoptotic signal in germ cells and the expression of Fas/FasL in testis increased correlatively in a time-dependent manner, reaching a maximum level on day 30.
Conclusion:
Fas system may initiate and regulate the germ cell apoptosis induced by testosterone undecanoate.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis / drug effects
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Contraceptive Agents, Male*
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Fas Ligand Protein
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Humans
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Macaca mulatta
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Male
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / physiology*
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Neuropeptides / genetics
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Neuropeptides / physiology*
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Oligospermia / chemically induced*
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Oligospermia / genetics
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Receptors, Tumor Necrosis Factor*
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Spermatozoa / pathology
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Testosterone* / analogs & derivatives*
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fas Receptor
Substances
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Contraceptive Agents, Male
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FAS protein, human
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FASLG protein, human
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Fas Ligand Protein
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Membrane Glycoproteins
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Neuropeptides
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Receptors, Tumor Necrosis Factor
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fas Receptor
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Testosterone
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testosterone undecanoate