Analysis of hypermutation of the 5' noncoding region in the BCL-6 gene

Chin Med J (Engl). 2001 Mar;114(3):286-90.

Abstract

Objective: To investigate BCL-6 gene mutations in Chinese populations with B-cell non-Hodgkin's lymphoma.

Methods: Polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE) and direct DNA sequencing were used to identify mutations in the 5' noncoding region of the BCL-6 gene in a total of 40 cases of diffuse large-cell lymphoma (DLCL) and follicular lymphoma (FL).

Results: Nine cases were found to have base substitutions. The incidence of BCL-6 gene mutation and the frequency of single-base changes were approximately 25.7% and (0.56-1.10) x 10(-2)/bp, respectively.

Conclusions: The 5' regulatory region of the BCL-6 gene undergoes frequent somatic hypermutation during lymphomagenesis and the identification of BCL-6 gene hypermutations provides a molecular marker for confirmatory diagnosis of B-NHL.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Flanking Region / genetics*
  • Base Sequence
  • DNA Mutational Analysis
  • DNA, Neoplasm / chemistry
  • DNA, Neoplasm / genetics
  • DNA-Binding Proteins / genetics*
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Lymphoma, Follicular / genetics*
  • Lymphoma, Large B-Cell, Diffuse / genetics*
  • Mutation
  • Point Mutation
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins c-bcl-6
  • Transcription Factors / genetics*

Substances

  • DNA, Neoplasm
  • DNA-Binding Proteins
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-6
  • Transcription Factors