Psoriasin expression in mammary epithelial cells in vitro and in vivo

Charlotta Enerbäck; Dale A Porter; Pankaj Seth; Dennis Sgroi; Justine Gaudet; Stanislawa Weremowicz; Cynthia C Morton; Stuart Schnitt; Robert L Pitts; Jason Stampl; Kerry Barnhart; Kornelia Polyak

Psoriasin expression in mammary epithelial cells in vitro and in vivo

Cancer Res. 2002 Jan 1;62(1):43-7.

Authors

Charlotta Enerbäck¹, Dale A Porter, Pankaj Seth, Dennis Sgroi, Justine Gaudet, Stanislawa Weremowicz, Cynthia C Morton, Stuart Schnitt, Robert L Pitts, Jason Stampl, Kerry Barnhart, Kornelia Polyak

Affiliation

¹ Department of Adult Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA.

PMID: 11782356

Abstract

We determined, by serial analysis of gene expression (SAGE) analysis of normal and DCIS (ductal carcinoma in situ) mammary epithelial cells, that psoriasin and several other genes implicated in psoriasis are aberrantly expressed in high-grade, comedo DCIS. Real-time PCR, mRNA in situ hybridization, and immunohistochemical analysis of breast carcinomas confirmed that psoriasin is frequently overexpressed in estrogen receptor-negative tumors. To gain insight into regulatory pathways that control psoriasin expression, we developed polyclonal and monoclonal antibodies and investigated mechanisms that may account for elevated levels of psoriasin in DCIS. Here, we report that loss of attachment to extracellular matrix, growth factor deprivation, and confluent conditions dramatically up-regulate psoriasin expression in MCF10A mammary epithelial cells. All of these conditions are characteristic of high-grade DCIS and psoriatic skin lesions; therefore, the same mechanisms may be responsible for increased expression of psoriasin in vitro and in vivo.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Amino Acid Sequence
Biomarkers, Tumor / biosynthesis*
Biomarkers, Tumor / genetics
Biomarkers, Tumor / metabolism
Breast / cytology
Breast / metabolism*
Breast / physiology
Breast Neoplasms / genetics
Breast Neoplasms / metabolism*
Calcium-Binding Proteins / biosynthesis*
Calcium-Binding Proteins / genetics
Calcium-Binding Proteins / metabolism
Carcinoma in Situ / genetics
Carcinoma in Situ / metabolism*
Carcinoma, Ductal, Breast / genetics
Carcinoma, Ductal, Breast / metabolism*
Cytoplasm / metabolism
Epithelial Cells / cytology
Epithelial Cells / metabolism
Female
Gene Expression Profiling
Humans
Immunohistochemistry
Molecular Sequence Data
Psoriasis / genetics
Psoriasis / metabolism
Psoriasis / pathology
Reverse Transcriptase Polymerase Chain Reaction
S100 Calcium Binding Protein A7
S100 Proteins
Subcellular Fractions / metabolism
Tumor Cells, Cultured

Substances

Biomarkers, Tumor
Calcium-Binding Proteins
S100 Calcium Binding Protein A7
S100 Proteins
S100A7 protein, human