Objective: To analyze the polymorphisms of the AACT, ERAB and NACP genes in Han Chinese Alzheimer's disease (AD) patients.
Methods: Denaturant gradient gel electrophoresis (DGGE) was used to scan all DNA fragments of 3 genes mentioned above in AD group (15 cases) and elderly control group (241 cases).
Results: A25G and G39A substitutions were found in the AACT gene. The gene frequency of A25G was 80% in the AD group and 47% in the control group (P < 0.05). The gene frequency of G39A genotype was 13% in the AD group and 0.4% in the control group (P < 0.01). The allele (39) frequencies were also significantly different between the two groups (P < 0.01). We did not find any points with polymorphism in the ERAB and NACP genes.
Conclusion: A25G and G39A substitutions in the AACT gene are probably one of the risk factors to AD in Han Chinese.