Abstract
Tissue factor pathway inhibitor (TFPI) is a protease inhibitor that regulates tissue factor (TF)--initiated coagulation. We report here that cardiomyocytes express TFPI and the expression could be increased by Interleukin-1(IL-1beta). The TFPI expression in cardiomyocytes was confirmed by Northern blotting with rat cardiomyocytes and also by immunostaining with anti-TFPI antibody on human heart specimens from patients either with sarcoidosis, myocarditis or myocardial infarction. The regulation of TFPI expression in cardiomyocytes differs from that in endothelial cells because TFPI expression is not induced in human endothelial cells by IL-1beta.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Northern
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Endothelium, Vascular / cytology
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Endothelium, Vascular / metabolism
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Humans
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Immunohistochemistry
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Interleukin-1 / pharmacology*
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Lipoproteins / biosynthesis*
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Lipoproteins / drug effects
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Lipoproteins / genetics
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Muscle, Smooth, Vascular / cytology
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Muscle, Smooth, Vascular / metabolism
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Myocardium / cytology
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Myocardium / metabolism*
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RNA, Messenger / drug effects
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RNA, Messenger / metabolism
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Rats
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Rats, Sprague-Dawley
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Thromboplastin / genetics
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Thromboplastin / metabolism
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Umbilical Veins / cytology
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Up-Regulation / drug effects*
Substances
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Interleukin-1
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Lipoproteins
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RNA, Messenger
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lipoprotein-associated coagulation inhibitor
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Thromboplastin