Frequency distribution of angiotensin-converting enzyme (ACE) gene genotypes and alleles was studied in patients with ischemic heart disease (IHD) and healthy subjects of Kazakh and Yugur nationalities. Polymorphism of ACE gene was determined by means of polymerase chain reaction. 88 IHD and 72 healthy Kazakhs and 70 and 70 Yugurs, respectively, were examined. Incidence of DD genotype in the studied groups was the least. This agrees with the literature data about low occurrence of genotype DD in Asian nationalities. Frequency of ACE gene genotypes in IHD and healthy subjects of Kazakh and Yugur nationalities do not differ significantly. Thus, polymorphism of ACE gene is unrelated to IHD in the examinees.