Abstract
Calcium/calmodulin-dependent serine protein kinase (CASK) showed overexpression in human esophageal carcinoma by suppression subtractive hybridization. The upregulation of CASK gene and its target gene Reelin in human esophageal carcinoma tissues versus corresponding normal tissues was revealed by reverse transcription polymerase chain reaction (RT-PCR), immunohistochemistry or Western blot. Moreover, RT-PCR results indicated that the expression patterns of CASK and Reelin in human gastric carcinoma and colon carcinoma were different with those in esophageal carcinoma. Therefore, it suggested that CASK and Reelin were associated with tumorigenesis of esophagus and they were co-upregulated in human esophageal carcinoma.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Blotting, Western
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Calcium-Calmodulin-Dependent Protein Kinases*
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Carcinoma, Squamous Cell / genetics
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Carcinoma, Squamous Cell / metabolism*
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Carcinoma, Squamous Cell / pathology
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Cell Adhesion Molecules, Neuronal / genetics*
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Cell Adhesion Molecules, Neuronal / metabolism
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Colonic Neoplasms / genetics
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Colonic Neoplasms / metabolism
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Colonic Neoplasms / pathology
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Esophageal Neoplasms / genetics
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Esophageal Neoplasms / metabolism*
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Esophageal Neoplasms / pathology
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Extracellular Matrix Proteins / genetics*
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Extracellular Matrix Proteins / metabolism
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Guanylate Kinases
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Humans
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Immunoenzyme Techniques
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Nerve Tissue Proteins
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Nucleoside-Phosphate Kinase / genetics*
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Nucleoside-Phosphate Kinase / metabolism
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RNA, Messenger / metabolism
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Reelin Protein
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Reverse Transcriptase Polymerase Chain Reaction
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Serine Endopeptidases
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Stomach Neoplasms / genetics
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Stomach Neoplasms / metabolism
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Stomach Neoplasms / pathology
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Up-Regulation
Substances
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Cell Adhesion Molecules, Neuronal
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Extracellular Matrix Proteins
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Nerve Tissue Proteins
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RNA, Messenger
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Reelin Protein
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CASK kinases
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Calcium-Calmodulin-Dependent Protein Kinases
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Nucleoside-Phosphate Kinase
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Guanylate Kinases
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RELN protein, human
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Serine Endopeptidases