Expression of transcription factor AML-2 (RUNX3, CBF(alpha)-3) is induced by Epstein-Barr virus EBNA-2 and correlates with the B-cell activation phenotype

J Virol. 2002 May;76(10):4919-27. doi: 10.1128/jvi.76.10.4919-4927.2002.

Abstract

To identify cell proteins regulated by the Epstein-Barr virus (EBV) transcription factor EBNA-2, we analyzed a cell line with conditional EBNA-2 activity by using microarray expression profiling. This led to the identification of two novel target genes induced by EBNA-2. The first of these, interleukin-16, is an immunomodulatory cytokine involved in the regulation of CD4 T cells. The second, AML-2, is a member of the Runt domain family of transcription factors. Quiescent B cells initially expressed AML-1 but, 48 h after virus infection, the levels of AML-1 decreased dramatically, whereas the amount of AML-2 protein increased. Analysis of a panel of B-cell lines indicated that AML-2 expression is normally predominant in EBV latency III, whereas AML-1 is associated with EBV latency I or EBV-negative cells. The AML genes are the first example of cell transcription factors whose expression correlates with the latency I/III phenotype.

Publication types

  • Comparative Study

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • B-Lymphocytes / metabolism
  • B-Lymphocytes / virology*
  • Burkitt Lymphoma
  • Carrier Proteins / metabolism
  • Cell Line
  • Cell Transformation, Viral / genetics*
  • Cells, Cultured
  • Core Binding Factor Alpha 2 Subunit
  • Core Binding Factor Alpha 3 Subunit
  • Cytoskeletal Proteins
  • DNA-Binding Proteins / biosynthesis*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Epstein-Barr Virus Nuclear Antigens / metabolism*
  • Gene Expression Profiling
  • Herpesvirus 4, Human*
  • Humans
  • Interleukin-16 / metabolism
  • Intracellular Signaling Peptides and Proteins
  • LIM Domain Proteins
  • Lymphocyte Activation / genetics
  • Oligonucleotide Array Sequence Analysis
  • Phenotype
  • Proto-Oncogene Proteins*
  • Transcription Factors / biosynthesis*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Tumor Cells, Cultured
  • Virus Latency / genetics

Substances

  • Adaptor Proteins, Signal Transducing
  • Carrier Proteins
  • Core Binding Factor Alpha 2 Subunit
  • Core Binding Factor Alpha 3 Subunit
  • Cytoskeletal Proteins
  • DNA-Binding Proteins
  • Epstein-Barr Virus Nuclear Antigens
  • Interleukin-16
  • Intracellular Signaling Peptides and Proteins
  • LIM Domain Proteins
  • PDLIM7 protein, human
  • Proto-Oncogene Proteins
  • RUNX1 protein, human
  • Runx3 protein, human
  • Transcription Factors