Abstract
Hypoxia within head and neck squamous cell carcinoma (HNSCC) predicts a poor response to radiotherapy and poor prognosis. Hypoxia-inducible factor (HIF)-1 and HIF-2 are nuclear transcription factors that regulate the cellular response to hypoxia and are important for solid tumor growth and survival. Overexpression of HIF-1alpha and HIF-2alpha was demonstrated in three HNSCC cell lines under hypoxia and tumor tissue versus normal tissue (n = 20, HIF-1alpha, P = 0.023; HIF-2alpha, P = 0.013). On immunostaining, HIF-1alpha and HIF-2alpha expression were localized to tumor nuclei; HIF-2alpha expression was also seen in tumor-associated macrophages. Expression of HIF-1alpha in surgically treated patients with HNSCC (n = 79) was associated with improved disease-free survival (P = 0.016) and overall survival (P = 0.027).
MeSH terms
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Adolescent
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Adult
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Aged
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Aged, 80 and over
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Basic Helix-Loop-Helix Transcription Factors
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Carcinoma, Squamous Cell / blood supply
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Carcinoma, Squamous Cell / metabolism*
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Carcinoma, Squamous Cell / pathology
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Carcinoma, Squamous Cell / surgery
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Disease-Free Survival
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HeLa Cells
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Head and Neck Neoplasms / blood supply
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Head and Neck Neoplasms / metabolism*
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Head and Neck Neoplasms / pathology
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Head and Neck Neoplasms / surgery
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Humans
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Hypoxia-Inducible Factor 1, alpha Subunit
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Immunohistochemistry
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L-Lactate Dehydrogenase / metabolism
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Middle Aged
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Necrosis
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Survival Rate
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Trans-Activators / biosynthesis*
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Transcription Factors / biosynthesis*
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Treatment Outcome
Substances
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Basic Helix-Loop-Helix Transcription Factors
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HIF1A protein, human
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Hypoxia-Inducible Factor 1, alpha Subunit
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Trans-Activators
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Transcription Factors
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endothelial PAS domain-containing protein 1
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L-Lactate Dehydrogenase