Identification and characterization of the gene encoding the human phosphopantetheine adenylyltransferase and dephospho-CoA kinase bifunctional enzyme (CoA synthase)

Biochem J. 2002 Jul 1;365(Pt 1):13-8. doi: 10.1042/BJ20020569.

Abstract

The final two enzymes in the CoA biosynthetic pathway, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), are separate proteins in prokaryotes, but exist as a bifunctional enzyme in pig liver. In the present study we have obtained sequence information from purified pig-liver enzyme, and identified the corresponding cDNA in a number of species. The human gene localizes to chromosome 17q12-21 and contains regions with sequence similarity to the monofunctional Escherichia coli DPCK and PPAT. The recombinant 564-amino-acid human protein confirmed the associated transferase and kinase activities, and gave similar kinetic properties to the wild-type pig enzyme.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 17 / genetics
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Gene Expression
  • Humans
  • Kinetics
  • Liver / enzymology
  • Molecular Sequence Data
  • Nucleotidyltransferases / genetics*
  • Nucleotidyltransferases / metabolism
  • Phosphotransferases (Alcohol Group Acceptor) / genetics*
  • Phosphotransferases (Alcohol Group Acceptor) / metabolism
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Swine

Substances

  • DNA, Complementary
  • Recombinant Proteins
  • Phosphotransferases (Alcohol Group Acceptor)
  • dephospho-CoA kinase
  • Nucleotidyltransferases
  • pantetheine-phosphate adenylyltransferase

Associated data

  • GENBANK/AY094602
  • GENBANK/AY094603