Abstract
Fluorescence in situ hybridization (FISH) analysis in a case of infant acute monocytic leukemia M5 revealed a complex rearrangement between chromosomes 10 and 11, leading to the disruption of the MLL gene. Using two painting probes for chromosomes 10 and 11 and a specific probe for the MLL gene localized on 11q23, we observed a paracentric inversion of the 11q13-q23 fragment translocated to 10p12. Molecular analysis showed that AF10 localized on 10p12 was the fusion partner gene of MLL in this rearrangement (10;11). This report underlined the usefulness of FISH and molecular techniques in identifying complex rearrangements.
MeSH terms
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Bone Marrow Transplantation
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Chromosome Inversion*
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Chromosomes, Human, Pair 10 / genetics
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Chromosomes, Human, Pair 10 / ultrastructure*
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Chromosomes, Human, Pair 11 / genetics
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Chromosomes, Human, Pair 11 / ultrastructure*
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DNA-Binding Proteins / genetics*
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Histone-Lysine N-Methyltransferase
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Humans
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In Situ Hybridization, Fluorescence
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Infant
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Leukemia, Monocytic, Acute / genetics*
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Male
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Myeloid-Lymphoid Leukemia Protein
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Oncogene Proteins, Fusion / genetics*
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Proto-Oncogenes*
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Transcription Factors / genetics*
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Translocation, Genetic* / genetics
Substances
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DNA-Binding Proteins
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KMT2A protein, human
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MLLT10 protein, human
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Oncogene Proteins, Fusion
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Transcription Factors
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Myeloid-Lymphoid Leukemia Protein
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Histone-Lysine N-Methyltransferase