Molecular and fluorescence in situ hybridization analysis of a 10;11 rearrangement in a case of infant acute monocytic leukemia

Cancer Genet Cytogenet. 2002 Jun;135(2):187-91. doi: 10.1016/s0165-4608(01)00644-6.

Abstract

Fluorescence in situ hybridization (FISH) analysis in a case of infant acute monocytic leukemia M5 revealed a complex rearrangement between chromosomes 10 and 11, leading to the disruption of the MLL gene. Using two painting probes for chromosomes 10 and 11 and a specific probe for the MLL gene localized on 11q23, we observed a paracentric inversion of the 11q13-q23 fragment translocated to 10p12. Molecular analysis showed that AF10 localized on 10p12 was the fusion partner gene of MLL in this rearrangement (10;11). This report underlined the usefulness of FISH and molecular techniques in identifying complex rearrangements.

Publication types

  • Case Reports

MeSH terms

  • Bone Marrow Transplantation
  • Chromosome Inversion*
  • Chromosomes, Human, Pair 10 / genetics
  • Chromosomes, Human, Pair 10 / ultrastructure*
  • Chromosomes, Human, Pair 11 / genetics
  • Chromosomes, Human, Pair 11 / ultrastructure*
  • DNA-Binding Proteins / genetics*
  • Histone-Lysine N-Methyltransferase
  • Humans
  • In Situ Hybridization, Fluorescence
  • Infant
  • Leukemia, Monocytic, Acute / genetics*
  • Male
  • Myeloid-Lymphoid Leukemia Protein
  • Oncogene Proteins, Fusion / genetics*
  • Proto-Oncogenes*
  • Transcription Factors / genetics*
  • Translocation, Genetic* / genetics

Substances

  • DNA-Binding Proteins
  • KMT2A protein, human
  • MLLT10 protein, human
  • Oncogene Proteins, Fusion
  • Transcription Factors
  • Myeloid-Lymphoid Leukemia Protein
  • Histone-Lysine N-Methyltransferase