Abstract
We studied Cyclin D1 (CyD1) and CD23 mRNA expression with real-time quantitative reverse transcription polymerase chain reaction (RQ-PCR) method. CyD1 expression in peripheral blood of seven mantle cell lymphoma (MCL) patients was found to be 1305.4 times higher than in 24 B-chronic lymphocytic leukemia (CLL) patients. CD23 expression in CLL was found to be 54.8 times higher than in MCL. These differences were statistically significant, and no overlap was found in CyD1 expression intensities between MCL and CLL. RQ-PCR allows rapid, simple and accurate quantification of CyD1 and CD23 expression, even from small samples, and is thus useful for the diagnosis of MCL and CLL.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Aged, 80 and over
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CD5 Antigens / analysis
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Cyclin D1 / biosynthesis*
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Cyclin D1 / genetics
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Female
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Gene Expression Regulation, Leukemic*
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Gene Expression Regulation, Neoplastic*
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Genes, Immunoglobulin
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Humans
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Immunoglobulin Heavy Chains / genetics
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Leukemia, Lymphocytic, Chronic, B-Cell / diagnosis
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Leukemia, Lymphocytic, Chronic, B-Cell / genetics*
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Leukemia, Lymphocytic, Chronic, B-Cell / metabolism
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Lymphoma, Mantle-Cell / diagnosis
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Lymphoma, Mantle-Cell / genetics*
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Lymphoma, Mantle-Cell / metabolism
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Male
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Middle Aged
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Neoplasm Proteins / biosynthesis*
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Neoplasm Proteins / genetics
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Oncogene Proteins, Fusion / genetics
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Receptors, IgE / biosynthesis*
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Receptors, IgE / genetics
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Reproducibility of Results
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Reverse Transcriptase Polymerase Chain Reaction
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Sensitivity and Specificity
Substances
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CD5 Antigens
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Immunoglobulin Heavy Chains
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Neoplasm Proteins
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Oncogene Proteins, Fusion
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Receptors, IgE
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Cyclin D1