Abstract
We reported earlier that expression of Sox-4 was found to be elevated during prostaglandin (PG) A(2) and delta(12)-PGJ(2) induced apoptosis in human hepatocarcinoma Hep3B cells. In this study, the role of Sox-4 was examined using human Hep3B and HepG2 cell lines. Sox-4 induction by several apoptotic inducer such as A23187 (Ca(2+) ionophore) and etoposide (topoisomerase II inhibitor) and Sox-4 transfection into the cells were able to induce apoptosis as observed by the cellular DNA fragmentation. Antisense oligonucleotide of Sox-4 inhibited the induction of Sox-4 expression and blocked the formation of DNA fragmentation by PGA(2) and delta(12)-PGJ(2) in Hep3B and HepG2 cells. Sox-4-induced apoptosis was accompanied with caspase-1 activation indicating that caspase cascade was involved in this apoptotic pathway. These results indicate that Sox-4 is involved in Hep3B and HepG2 cells apoptosis as an important apoptotic mediator.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis / drug effects*
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Blotting, Western
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Calcimycin / pharmacology
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Caspase 1 / metabolism
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Caspase Inhibitors
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Etoposide / pharmacology
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Gene Expression Regulation, Neoplastic / drug effects
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High Mobility Group Proteins / genetics
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High Mobility Group Proteins / metabolism*
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Humans
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Liver Neoplasms / enzymology
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Liver Neoplasms / metabolism
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Liver Neoplasms / pathology
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Oligopeptides / pharmacology
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Prostaglandin D2 / analogs & derivatives*
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Prostaglandin D2 / pharmacology*
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Prostaglandins A / pharmacology*
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SOXC Transcription Factors
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Trans-Activators / genetics
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Trans-Activators / metabolism*
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Transfection
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Tumor Cells, Cultured
Substances
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Caspase Inhibitors
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High Mobility Group Proteins
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Oligopeptides
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Prostaglandins A
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SOX4 protein, human
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SOXC Transcription Factors
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Trans-Activators
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L 709049
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Calcimycin
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9-deoxy-delta-9-prostaglandin D2
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Etoposide
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Caspase 1
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prostaglandin A2
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Prostaglandin D2