Abstract
Human chromosome translocation t(12;21)(p12;q22) is the most frequent chromosome rearrangement in childhood B-lineage acute lymphoblastic leukemia (ALL), and produces the TEL/AML1 fusion protein. The chimeric protein, TEL/AML1 contains the first 336 amino acids of TEL that is linked to residues 21-480 of AML1 and the fusion protein is generally known as a transcription repressor to the various target genes. Furthermore, TEL/AML1 has been shown to interfere with AML1-mediated transactivation on the CR1 gene. To understand the mechanism of the TEL/AML1-mediated repression, we used transient-transfection assay and immunofluorescence to monitor subcellular localization of TEL/AML1. Here, we show that TEL/AML1 is localized in the cytoplasm and the transcriptional activities of CR1 promoter are affected by the subcellular localization of TEL/AML1 fusion protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Core Binding Factor Alpha 2 Subunit
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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DNA-Binding Proteins / pharmacology
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ETS Translocation Variant 6 Protein
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Humans
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Protein Structure, Tertiary
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Proto-Oncogene Proteins c-ets
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Proto-Oncogene Proteins*
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Receptors, Complement 3b / genetics*
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / metabolism
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Recombinant Fusion Proteins / pharmacology
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Repressor Proteins / chemistry
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Repressor Proteins / genetics
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Repressor Proteins / metabolism
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Repressor Proteins / pharmacology
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Statistics as Topic
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Subcellular Fractions
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Transcription Factors / chemistry
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Transcription Factors / pharmacology
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Transcriptional Activation / drug effects*
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Tumor Cells, Cultured
Substances
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Core Binding Factor Alpha 2 Subunit
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DNA-Binding Proteins
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-ets
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RUNX1 protein, human
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Receptors, Complement 3b
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Recombinant Fusion Proteins
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Repressor Proteins
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Transcription Factors