Abstract
In L6E9 skeletal muscle cells ternary complex factor (TCF) Elk-1 expression increased with the onset of skeletal muscle differentiation, whereas its activation decreased as a function of differentiation. Its expression was predominantly restricted to cytoplasm and activated ones were predominantly restricted to the nucleus of the differentiated cells. Inhibition of ERK-1/-2 activities by PD098059 resulted into significant reduction in Elk-1 expression and phosphorylation during differentiation. In contrast, inhibition of p38 mitogen-activated protein kinase (MAPK) enhanced Elk-1 expression and activation, thereby mediating inhibition of skeletal muscle differentiation. Overexpression of inactive mutant Elk-1 enhanced differentiation. Data suggest that ERK-1/-2 and p38 MAPK activities modulate Elk-1 expression and activation to regulate skeletal muscle differentiation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Differentiation / drug effects
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Cell Differentiation / physiology
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Cell Nucleus / drug effects
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Cell Nucleus / metabolism
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Cells, Cultured
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Cytoplasm / drug effects
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Cytoplasm / metabolism
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DNA-Binding Proteins*
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Enzyme Inhibitors / pharmacology
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Flavonoids / pharmacology
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Imidazoles / pharmacology
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Mitogen-Activated Protein Kinase 1 / antagonists & inhibitors
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Mitogen-Activated Protein Kinase 1 / metabolism
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Mitogen-Activated Protein Kinase 3
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Mitogen-Activated Protein Kinases / antagonists & inhibitors
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Mitogen-Activated Protein Kinases / metabolism
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Muscle, Skeletal / cytology*
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Muscle, Skeletal / drug effects
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Muscle, Skeletal / metabolism*
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Mutation
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Phosphorylation
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Proto-Oncogene Proteins / drug effects
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins / metabolism*
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Pyridines / pharmacology
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Rats
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Transcription Factors*
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ets-Domain Protein Elk-1
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p38 Mitogen-Activated Protein Kinases
Substances
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DNA-Binding Proteins
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Elk1 protein, rat
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Enzyme Inhibitors
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Flavonoids
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Imidazoles
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Proto-Oncogene Proteins
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Pyridines
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Transcription Factors
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ets-Domain Protein Elk-1
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Mitogen-Activated Protein Kinase 1
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Mitogen-Activated Protein Kinase 3
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Mitogen-Activated Protein Kinases
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p38 Mitogen-Activated Protein Kinases
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SB 203580
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2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one