Abstract
The in vitro invasiveness of human breast cancer cell lines was compared with their reported tumorigenicity in vivo, increasing from MCF7, MDA-MB468, MDA-MB231 to MDA-MB435 cells. The invasiveness roughly corresponded to the tumorigenicity of the cell lines. The levels of cathepsin L mRNA and protein correlated with the invasiveness of the cells. Stefin A protein decreased with the invasiveness and the reported tumorigenicity, whereas stefin B protein was significantly lower in all MDA-MB lines compared with the least invasive and tumorigenic MCF7 line. Our results suggest that the imbalance between cathepsin L and the stefins contributes to the development of a malignant cell phenotype.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Breast Neoplasms / enzymology*
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Breast Neoplasms / pathology
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Cathepsin L
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Cathepsins / antagonists & inhibitors
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Cathepsins / genetics
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Cathepsins / metabolism*
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Collagen / chemistry
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Cystatin A
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Cystatin B
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Cystatins / genetics
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Cystatins / metabolism*
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Cysteine Endopeptidases / genetics
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Cysteine Endopeptidases / metabolism*
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Cysteine Proteinase Inhibitors / genetics
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Cysteine Proteinase Inhibitors / metabolism*
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Disease Progression
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Drug Combinations
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Enzyme-Linked Immunosorbent Assay
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Female
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Humans
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In Vitro Techniques
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Laminin / chemistry
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Neoplasm Invasiveness
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Proteoglycans / chemistry
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RNA, Neoplasm / metabolism
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Tumor Cells, Cultured / metabolism*
Substances
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CSTB protein, human
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Cystatin A
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Cystatins
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Cysteine Proteinase Inhibitors
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Drug Combinations
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Laminin
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Proteoglycans
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RNA, Neoplasm
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matrigel
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CSTA protein, human
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Cystatin B
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Collagen
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Cathepsins
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Cysteine Endopeptidases
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CTSL protein, human
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Cathepsin L