Dominant expression of interleukin 10 but not interferon gamma in CD4(-)CD8(-)alphabetaT cells of autoimmune lymphoproliferative syndrome

Br J Haematol. 2002 Nov;119(2):535-8. doi: 10.1046/j.1365-2141.2002.03848.x.

Abstract

Cytokine expression in CD4-CD8- double-negative (DN) T cells of autoimmune lymphoproliferative syndrome (ALPS) was analysed. Two patients with DN alphabetaT-cell expansion showed higher serum interleukin 10 (IL-10) levels than one patient without it. Intracellular flow-cytometric analysis indicated the IL-10-expressing CD3+CD4-CD8- cells in patients with lymphoproliferation. Quantitative real-time polymerase chain reaction revealed approximately 100 times higher IL-10, but not interferon-gamma or transforming growth factor-beta in DN than in single-positive T cells. IL-10 was exclusively expressed in DN alphabeta but not (gamma)(delta)T cells. Circulating DN alphabetaT cells may constitutively express IL-10 and contribute to the ALPS phenotype.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autoimmune Diseases / immunology*
  • CD3 Complex / immunology
  • Case-Control Studies
  • Child
  • HLA-DR Antigens / immunology
  • Humans
  • Interferon-gamma / genetics
  • Interferon-gamma / immunology*
  • Interleukin-10 / genetics
  • Interleukin-10 / immunology*
  • Lymphoproliferative Disorders / immunology*
  • RNA, Messenger / analysis
  • Receptors, Antigen, T-Cell, alpha-beta*
  • Reverse Transcriptase Polymerase Chain Reaction
  • T-Lymphocyte Subsets / immunology*
  • Transforming Growth Factor beta / genetics
  • Transforming Growth Factor beta / immunology

Substances

  • CD3 Complex
  • HLA-DR Antigens
  • RNA, Messenger
  • Receptors, Antigen, T-Cell, alpha-beta
  • Transforming Growth Factor beta
  • Interleukin-10
  • Interferon-gamma