Abstract
Transforming growth factor (TGF)-beta-inducible gene-h3 (betaig-h3) product is a secreted protein that is induced by TGF-beta in several cell types and implicated in various tissue pathologies. The aims of this study were to determine the effect of TGF-beta1 on betaig-h3 expression in cultured astrocytes and to examine whether betaig-h3 is expressed in the brain after traumatic injury. The results showed that betaig-h3 mRNA and protein increased in response to TGF-beta1 in U87 human astrocytoma cells and mouse cortical astrocytes. Treatment with other cytokines, including tumor necrosis factor-alpha and fibroblast growth factor-2, did not enhance the expression of betaig-h3 in astrocytes. betaig-h3 was significantly expressed in reactive astrocytes at the site of a stab wound in the cerebral cortex of adult rats. These results provide an insight into understanding a novel role for betaig-h3 protein in the response of astrocytes to brain injury.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Astrocytes / drug effects
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Astrocytes / metabolism*
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Brain Injuries / genetics
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Brain Injuries / metabolism*
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Dose-Response Relationship, Drug
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Extracellular Matrix Proteins*
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Gene Expression Regulation / drug effects
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Gene Expression Regulation / physiology
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Glial Fibrillary Acidic Protein / metabolism
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Gliosis / genetics
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Gliosis / metabolism*
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Humans
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Immunohistochemistry
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Neoplasm Proteins / genetics
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Neoplasm Proteins / metabolism*
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RNA, Messenger / drug effects
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RNA, Messenger / metabolism
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Rats
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Rats, Sprague-Dawley
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Transforming Growth Factor beta / metabolism*
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Transforming Growth Factor beta / pharmacology
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Transforming Growth Factor beta1
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Tumor Cells, Cultured
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Up-Regulation / drug effects
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Up-Regulation / physiology
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Wound Healing / drug effects
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Wound Healing / genetics*
Substances
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Extracellular Matrix Proteins
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Glial Fibrillary Acidic Protein
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Neoplasm Proteins
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RNA, Messenger
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TGFB1 protein, human
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Tgfb1 protein, mouse
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Tgfb1 protein, rat
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Transforming Growth Factor beta
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Transforming Growth Factor beta1
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betaIG-H3 protein