Abstract
The host factor LSF represses the human immunodeficiency virus type 1 long terminal repeat (LTR) by mediating recruitment of histone deacetylase. We show that pyrrole-imidazole polyamides targeted to the LTR can specifically block LSF binding both in vitro and within cells via direct access to chromatin, resulting in increased LTR expression.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Cell Line
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DNA, Viral / genetics
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DNA-Binding Proteins / metabolism
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Erythroid-Specific DNA-Binding Factors
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Gene Expression / drug effects
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Genes, Viral / drug effects
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HIV Infections / genetics
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HIV Infections / metabolism
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HIV Infections / virology
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HIV Long Terminal Repeat / drug effects*
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HIV-1 / drug effects*
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HIV-1 / genetics*
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HeLa Cells
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Histone Deacetylases / metabolism
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Humans
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Imidazoles / chemistry
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Imidazoles / pharmacology
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In Vitro Techniques
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Molecular Sequence Data
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Nylons / chemistry
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Nylons / pharmacology*
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Pyrroles / chemistry
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Pyrroles / pharmacology
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RNA-Binding Proteins
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Transcription Factors / metabolism
Substances
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DNA, Viral
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DNA-Binding Proteins
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Erythroid-Specific DNA-Binding Factors
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Imidazoles
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Nylons
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Pyrroles
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RNA-Binding Proteins
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TFCP2 protein, human
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Transcription Factors
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Histone Deacetylases